Evidence map›Paper›PMID 25344639›Full record

ArticleNature methods2014

Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex.

Arnaud Muller, Victory Joseph, Paul A Slesinger, David Kleinfeld

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 146 citations in OpenAlex.

  1. Article
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  6. Genetically encoded sensors illuminateIBRO neuroscience reports · 2025
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  13. Apoptosis signal-regulating kinase 1 (Frontiers in cellular neuroscience · 2024
    Article
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  15. Review
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  17. Review
  18. Article
  19. Review
  20. A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Arnaud MullerDepartment of Physics, University of California at San Diego, La Jolla, California, USA.
Victory JosephGraduate Program in Neurosciences, University of California at San Diego, La Jolla, California, USA.
Paul A Slesinger1] Graduate Program in Neurosciences, University of California at San Diego, La Jolla, California, USA. [2] Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
David Kleinfeld1] Department of Physics, University of California at San Diego, La Jolla, California, USA. [2] Graduate Program in Neurosciences, University of California at San Diego, La Jolla, California, USA. [3] Section of Neurobiology, University of California at San Diego, La Jolla, California, USA. [4] Department of Electrical and Computer Engineering, University of California at San Diego, La Jolla, California, USA.
University of California, San Diego · US

Funding

Manipulating Neural Tissue With Ultra-Short Laser PulsesR01EB003832 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KAUFHOLD, JOHN, KLEINFELD, DAVID · 2005 to 2014
$8.2M
CNiFERs Cell-based Neurotransmitter Fluorescent Engineered ReportersR01DA029706 · NIDA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KLEINFELD, DAVID · 2010 to 2014
$2.3M
Defining the Logic of Neurovascular Signaling in the BrainDP1NS082097 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KLEINFELD, DAVID · 2012 to 2014
$2.3M
Defining the Logic of Neurovascular Signaling in the BrainDP1OD006831 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KLEINFELD, DAVID · 2010 to 2011
$1.5M
NIBIB NIH HHS EB003832NIBIB NIH HHS R01 EB003832NIDA NIH HHS DA007315NIDA NIH HHS DA029706NIDA NIH HHS R01 DA029706NIH HHS DP1 OD006831NINDS NIH HHS DP1 NS082097
6 · The paper itself

Abstract

The neuronal coding of stimulus-to-action sequences is believed to involve the release of dopamine (DA) and norepinephrine (NE). The electrochemical similarity of these monoamines, however, confounds real-time measurements of their release. Here we report cell-based neurotransmitter fluorescent engineered reporters (CNiFERs) that use the specificity of G protein-coupled receptors (GPCRs) to discriminate nanomolar concentrations of DA and NE. CNiFERs were implanted into the frontal cortex of mice to measure the timing of neurotransmitter release during classical conditioning with the use of two-photon microscopy. The onset of DA release correlated with that of licking and shifted from the time of the reward toward that of the cue upon conditioning. In contrast, concurrent release of NE did not correlate with licking or the cue. This generation of CNiFERs provides unique tools to assess the release of monoamines. The molecular design of these CNiFERs may be generalized to realize CNiFERs for any molecule that activates a GPCR.

Indexed as

Behavior, AnimalAnimalsCerebral CortexDopamineFluorescence Resonance Energy TransferFluorescent DyesLearningMiceNorepinephrineReceptors, G-Protein-CoupledSynaptic TransmissionDopamineFluorescent DyesNorepinephrineReceptors, G-Protein-Coupled

Identifiers

PMID25344639
PMCPMC4245316
OpenAlexW2123300469

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.