ArticlePlant molecular biology2015
CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
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Who cites it
141 citing papers in PubMed, 337 citations in OpenAlex.
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Comparative analysis of chloroplast genomes reveals molecular evolution and phylogenetic relationships in Styrax (Styracaceae).BMC plant biology · 2026Article
- Decapitation Rapidly Triggers Axillary Bud Release via Regulatory Network Reprogramming inPlants (Basel, Switzerland) · 2025Article
- Targeted mutagenesis ofaBIOTECH · 2025Article
- A novel recombinant CRISPR/Cas9 vector system for genome editing in plants.Transgenic research · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Knocking out artificially selected gene GmAOC4TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cas system.Molecular horticulture · 2025Article
- Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing ofFrontiers in plant science · 2025Article
- Integration of CRISPR/Cas9 with multi-omics technologies to engineer secondary metabolite productions in medicinal plant: Challenges and Prospects.Functional & integrative genomics · 2024Review
- Advancements and Future Prospects of CRISPR-Cas-Based Population Replacement Strategies in Insect Pest Management.Insects · 2024Review
- Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals.Frontiers in plant science · 2024Review
- Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production.Frontiers in genome editing · 2024Review
- CRISPR/Cas9-gene editing approaches in plant breeding.GM crops & food · 2023Review
- CRISPR/Cas9 Editing Sites Identification and Multi-Elements Association Analysis inInternational journal of molecular sciences · 2023Article
- Development of an Agrobacterium-delivered codon-optimized CRISPR/Cas9 system for chickpea genome editing.Protoplasma · 2023Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Role ofHorticulture research · 2023Article
- Using A Protoplast Transformation System to Enable Functional Studies inInternational journal of molecular sciences · 2023Article
- Effects of editing DFR genes on flowers, leaves, and roots of tobacco.BMC plant biology · 2023Article
81 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing is one of the most powerful tools for revealing gene function and improving crop plants. Recently, RNA-guided genome editing using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) system has been used as a powerful and efficient tool for genome editing in various organisms. Here, we report genome editing in tobacco (Nicotiana tabacum) mediated by the CRISPR/Cas9 system. Two genes, NtPDS and NtPDR6, were used for targeted mutagenesis. First, we examined the transient genome editing activity of this system in tobacco protoplasts, insertion and deletion (indel) mutations were observed with frequencies of 16.2-20.3% after transfecting guide RNA (gRNA) and the nuclease Cas9 in tobacco protoplasts. The two genes were also mutated using multiplexing gRNA at a time. Additionally, targeted deletions and inversions of a 1.8-kb fragment between two target sites in the NtPDS locus were demonstrated, while indel mutations were also detected at both the sites. Second, we obtained transgenic tobacco plants with NtPDS and NtPDR6 mutations induced by Cas9/gRNA. The mutation percentage was 81.8% for NtPDS gRNA4 and 87.5% for NtPDR6 gRNA2. Obvious phenotypes were observed, etiolated leaves for the psd mutant and more branches for the pdr6 mutant, indicating that highly efficient biallelic mutations occurred in both transgenic lines. No significant off-target mutations were obtained. Our results show that the CRISPR/Cas9 system is a useful tool for targeted mutagenesis of the tobacco genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.