ArticlePlant physiology2014
An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Observing the invisible: X-ray CT for plant-microbe interactions.The New phytologist · 2026Review
- Optical clearing of apple tissues for in vivo imaging of the pathogenic behavior of the fungus Botryosphaeria dothidea on host surfaces.Applied microscopy · 2025Article
- A Method to Visualize Cell Proliferation ofPlant direct · 2025Article
- A neutral invertase controls cell division besides hydrolysis of sucrose for nutrition during germination and seed setting in rice.iScience · 2024Article
- Clearing techniques for deeper imaging of plants and plant-microbe interactions.Applied microscopy · 2024Review
- Clearing of Vascular Tissue in Arabidopsis thaliana for Reporter Analysis of Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana.Plant reproduction · 2022Article
- Development of Microscopic Techniques for the Visualization of Plant-Root-Knot Nematode Interaction.Plants (Basel, Switzerland) · 2022Article
- Improved clearing method contributes to deep imaging of plant organs.Communications biology · 2022Article
- Means to Quantify Vascular Cell File Numbers in Different Tissues.Methods in molecular biology (Clifton, N.J.) · 2022Article
- ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.Plant & cell physiology · 2021Article
- Early stages of legume-rhizobia symbiosis are controlled by ABCG-mediated transport of active cytokinins.Nature plants · 2021Article
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- New methods for confocal imaging of infection threads in crop and model legumes.Plant methods · 2021Article
- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- TheFrontiers in plant science · 2021Article
- A Sight on Single-Cell Transcriptomics in Plants Through the Prism of Cell-Based Computational Modeling Approaches: Benefits and Challenges for Data Analysis.Frontiers in genetics · 2021Review
- Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.AoB PLANTS · 2020Article
- Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize.Nature communications · 2020Article
- Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.Neuron · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We report on a nondestructive clearing technique that enhances transmission of light through specimens from diverse plant species, opening unique opportunities for microscope-enabled plant research. After clearing, plant organs and thick tissue sections are amenable to deep imaging. The clearing method is compatible with immunocytochemistry techniques and can be used in concert with common fluorescent probes, including widely adopted protein tags such as GFP, which has fluorescence that is preserved during the clearing process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.