ArticleRNA (New York, N.Y.)2014
Distinctive profiles of small RNA couple inverted repeat-induced post-transcriptional gene silencing with endogenous RNA silencing pathways in Arabidopsis.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- Small RNA, big defence: Early epigenetic responses to genetic invasion.Quantitative plant biology · 2025Review
- Article
- Plant gene silencing signals move from the phloem to influence gene expression in shoot apical meristems.BMC plant biology · 2022Article
- Perspective: 50 years of plant chromosome biology.Plant physiology · 2021Article
- The Whys and Wherefores of Transitivity in Plants.Frontiers in plant science · 2020Review
- Detailed insight into the dynamics of the initial phases of de novo RNA-directed DNA methylation in plant cells.Epigenetics & chromatin · 2019Article
- PCIR: a database of Plant Chloroplast Inverted Repeats.Database : the journal of biological databases and curation · 2019Article
- The Prevalence and Evolutionary Conservation of Inverted Repeats in Proteobacteria.Genome biology and evolution · 2018Article
- Enhanced Host-Parasite Resistance Based on Down-Regulation ofFrontiers in plant science · 2017Article
- Genome-Wide Architecture of Disease Resistance Genes in Lettuce.G3 (Bethesda, Md.) · 2015Article
- Post-transcriptional gene silencing triggered by sense transgenes involves uncapped antisense RNA and differs from silencing intentionally triggered by antisense transgenes.Nucleic acids research · 2015Article
- A Genome-wide Combinatorial Strategy Dissects Complex Genetic Architecture of Seed Coat Color in Chickpea.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The experimental induction of RNA silencing in plants often involves expression of transgenes encoding inverted repeat (IR) sequences to produce abundant dsRNAs that are processed into small RNAs (sRNAs). These sRNAs are key mediators of post-transcriptional gene silencing (PTGS) and determine its specificity. Despite its application in agriculture and broad utility in plant research, the mechanism of IR-PTGS is incompletely understood. We generated four sets of 60 Arabidopsis plants, each containing IR transgenes expressing different configurations of uidA and CHALCONE Synthase (At-CHS) gene fragments. Levels of PTGS were found to depend on the orientation and position of the fragment in the IR construct. Deep sequencing and mapping of sRNAs to corresponding transgene-derived and endogenous transcripts identified distinctive patterns of differential sRNA accumulation that revealed similarities among sRNAs associated with IR-PTGS and endogenous sRNAs linked to uncapped mRNA decay. Detailed analyses of poly-A cleavage products from At-CHS mRNA confirmed this hypothesis. We also found unexpected associations between sRNA accumulation and the presence of predicted open reading frames in the trigger sequence. In addition, strong IR-PTGS affected the prevalence of endogenous sRNAs, which has implications for the use of PTGS for experimental or applied purposes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.