ArticleApplied and environmental microbiology2015
A novel DNA-binding protein, PhaR, plays a central role in the regulation of polyhydroxyalkanoate accumulation and granule formation in the haloarchaeon Haloferax mediterranei.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 52 citations in OpenAlex.
- Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.PLoS biology · 2026Article
- Heterologous expression and characterization of polyhydroxyalkanoate synthase genes from haloarchaeal strains inFrontiers in microbiology · 2026Article
- Adaptive laboratory evolution under oxidative stress in lactococcus lactis subsp. lactis LM1009: transcriptomic remodeling and functional enhancement.World journal of microbiology & biotechnology · 2025Article
- Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the ExtremophileMarine drugs · 2025Article
- Production of Poly(3-hydroxybutyrate-Bioengineering (Basel, Switzerland) · 2024Article
- Isolation and characterization of aApplied and environmental microbiology · 2024Article
- Bioplastic Production from Agri-Food Waste through the Use ofMicroorganisms · 2024Review
- Unveiling the repressive mechanism of a PPS-like regulator (PspR) in polyhydroxyalkanoates biosynthesis network.Applied microbiology and biotechnology · 2024Article
- Structural bases for aspartate recognition and polymerization efficiency of cyanobacterial cyanophycin synthetase.Nature communications · 2022Article
- An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis.Microbial biotechnology · 2022Review
- Reprogramming the endogenous type I CRISPR-Cas system for simultaneous gene regulation and editing inmLife · 2022Article
- Study on the production of high 3HV content PHBVFrontiers in microbiology · 2022Article
- Divergent degeneration of creA antitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins.Nucleic acids research · 2021Article
- Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux.Communications biology · 2021Article
- ORF4 of the Temperate Archaeal Virus SNJ1 Governs the Lysis-Lysogeny Switch and Superinfection Immunity.Journal of virology · 2020Article
- Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory.Microbial cell factories · 2020Review
- Global Proteomic Analysis Reveals High Light Intensity Adaptation Strategies and Polyhydroxyalkanoate Production inFrontiers in microbiology · 2020Article
- Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica.Nucleic acids research · 2019Article
- EngineeringFrontiers in microbiology · 2018Article
- Mutagenesis ofFrontiers in microbiology · 2018Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyhydroxyalkanoates (PHAs) are synthesized and assembled as PHA granules that undergo well-regulated formation in many microorganisms. However, this regulation remains unclear in haloarchaea. In this study, we identified a PHA granule-associated regulator (PhaR) that negatively regulates the expression of both its own gene and the granule structural gene phaP in the same operon (phaRP) in Haloferax mediterranei. Chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) assays demonstrated a significant interaction between PhaR and the phaRP promoter in vivo. Scanning mutagenesis of the phaRP promoter revealed a specific cis-element as the possible binding position of the PhaR. The haloarchaeal homologs of the PhaR contain a novel conserved domain that belongs to a swapped-hairpin barrel fold family found in AbrB-like proteins. Amino acid substitution indicated that this AbrB-like domain is critical for the repression activity of PhaR. In addition, the phaRP promoter had a weaker activity in the PHA-negative strains, implying a function of the PHA granules in titration of the PhaR. Moreover, the H. mediterranei strain lacking phaR was deficient in PHA accumulation and produced granules with irregular shapes. Interestingly, the PhaR itself can promote PHA synthesis and granule formation in a PhaP-independent manner. Collectively, our results demonstrated that the haloarchaeal PhaR is a novel bifunctional protein that plays the central role in the regulation of PHA accumulation and granule formation in H. mediterranei.
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