ArticleApplied and environmental microbiology2015
Molecular and metabolic adaptations of Lactococcus lactis at near-zero growth rates.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 25 citations in OpenAlex.
- Global transcriptional regulation by the CodY gene: identification of critical compounds that enhance oxidative stress resistance in Lactobacillus delbrueckii subsp. Bulgaricus.BMC microbiology · 2025Article
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications inMicroorganisms · 2023Article
- The growth-survival trade-off is hard-wired in the Lactococcus lactis gene regulation network.Environmental microbiology reports · 2022Article
- Systems-Level Analysis of the Global Regulatory Mechanism of CodY in Lactococcus lactis Metabolism and Nisin Immunity Modulation.Applied and environmental microbiology · 2022Article
- Continuous Cultivation as a Tool Toward the Rational Bioprocess Development WithFrontiers in bioengineering and biotechnology · 2020Review
- A Novel Method for Long-Term Analysis of Lactic Acid and Ammonium Production in Non-growingFrontiers in bioengineering and biotechnology · 2020Article
- A Reproducible and Scalable Process for Manufacturing a Pfs48/45 BasedFrontiers in immunology · 2020Article
- Contribution of YthA, a PspC Family Transcriptional Regulator of Lactococcus lactis F44 Acid Tolerance and Nisin Yield: a Transcriptomic Approach.Applied and environmental microbiology · 2018Article
- The Influence of Viable Cells and Cell-Free Extracts ofFrontiers in microbiology · 2018Article
- Biosorption of silver cations onto Lactococcus lactis and Lactobacillus casei isolated from dairy products.PloS one · 2017Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Physiological and Transcriptional Responses of Different Industrial Microbes at Near-Zero Specific Growth Rates.Applied and environmental microbiology · 2015Review
- Genome-wide transcriptional responses to carbon starvation in nongrowing Lactococcus lactis.Applied and environmental microbiology · 2015Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper describes the molecular and metabolic adaptations of Lactococcus lactis during the transition from a growing to a near-zero growth state by using carbon-limited retentostat cultivation. Transcriptomic analyses revealed that metabolic patterns shifted between lactic- and mixed-acid fermentations during retentostat cultivation, which appeared to be controlled at the level of transcription of the corresponding pyruvate dissipation-encoding genes. During retentostat cultivation, cells continued to consume several amino acids but also produced specific amino acids, which may derive from the conversion of glycolytic intermediates. We identify a novel motif containing CTGTCAG in the upstream regions of several genes related to amino acid conversion, which we propose to be the target site for CodY in L. lactis KF147. Finally, under extremely low carbon availability, carbon catabolite repression was progressively relieved and alternative catabolic functions were found to be highly expressed, which was confirmed by enhanced initial acidification rates on various sugars in cells obtained from near-zero-growth cultures. The present integrated transcriptome and metabolite (amino acids and previously reported fermentation end products) study provides molecular understanding of the adaptation of L. lactis to conditions supporting low growth rates and expands our earlier analysis of the quantitative physiology of this bacterium at near-zero growth rates toward gene regulation patterns involved in zero-growth adaptation.
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