ArticleApplied and environmental microbiology2015
Uptake of amino acids and their metabolic conversion into the compatible solute proline confers osmoprotection to Bacillus subtilis.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 75 citations in OpenAlex.
- Orthology-based comparative transcriptomic analysis reveals conserved osmoadaptive responses in Bacillus velezensis and Bacillus paralicheniformis.Archives of microbiology · 2026Article
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- Mechanistic insights into the antimicrobial activity of a novel essential oil blend againstAIMS microbiology · 2026Article
- Conserved accessory genes link a phylogenetically distinct Bacillus subtilis strain from Indian bekang to the Japanese natto clade.Scientific reports · 2025Article
- Time-series metatranscriptomics reveals differential salinity effects on the methanogenic food web in paddy soil.mSystems · 2025Article
- Bacterial osmoprotectants-a way to survive in saline conditions and potential crop allies.FEMS microbiology reviews · 2025Review
- Metabolic pathway engineering of high-salinity-induced overproduction of L-proline improves high-salinity stress tolerance of an ectoine-deficientApplied and environmental microbiology · 2024Article
- Transcriptome Profiles Reveal Key Regulatory Networks during Single and Multifactorial Stresses Coupled with Melatonin Treatment in Pitaya (International journal of molecular sciences · 2024Article
- Upregulated Palmitoleate and Oleate Production inMolecules (Basel, Switzerland) · 2024Article
- Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway.Microbial biotechnology · 2024Article
- Isolation and Genomics ofMicrobiology spectrum · 2023Article
- How To Deal with Toxic Amino Acids: the Bipartite AzlCD Complex Exports Histidine inJournal of bacteriology · 2022Article
- Stress-induced activation of the proline biosynthetic pathway in Bacillus subtilis: a population-wide and single-cell study of the osmotically controlled proHJ promoter.Microbial biotechnology · 2022Article
- Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana.Scientific reports · 2022Article
- Proline confers acid stress tolerance to Bacillus megaterium G18.Scientific reports · 2022Article
- Article
- The impact of PrsA over-expression on theFrontiers in microbiology · 2022Article
- L-Proline Synthesis Mutants ofFrontiers in microbiology · 2022Article
- Genomic Analysis ofFrontiers in microbiology · 2021Article
- Enhanced Glutamate Synthesis and Export by the Thermotolerant Emerging Industrial WorkhorseFrontiers in microbiology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The data presented here reveal a new facet of the physiological adjustment processes through which Bacillus subtilis can derive osmostress protection. We found that the import of proteogenic (Glu, Gln, Asp, Asn, and Arg) and of nonproteogenic (Orn and Cit) amino acids and their metabolic conversion into proline enhances growth under otherwise osmotically unfavorable conditions. Osmoprotection by amino acids depends on the functioning of the ProJ-ProA-ProH enzymes, but different entry points into this biosynthetic route are used by different amino acids to finally yield the compatible solute proline. Glu, Gln, Asp, and Asn are used to replenish the cellular pool of glutamate, the precursor for proline production, whereas Arg, Orn, and Cit are converted into γ-glutamic semialdehyde/Δ(1)-pyrroline-5-carboxylate, an intermediate in proline biosynthesis. The import of Glu, Gln, Asp, Asn, Arg, Orn, and Cit did not lead to a further increase in the size of the proline pool that is already present in osmotically stressed cells. Hence, our data suggest that osmoprotection of B. subtilis by this group of amino acids rests on the savings in biosynthetic building blocks and energy that would otherwise have to be devoted either to the synthesis of the proline precursor glutamate or of proline itself. Since glutamate is the direct biosynthetic precursor for proline, we studied its uptake and found that GltT, an Na(+)-coupled symporter, is the main uptake system for both glutamate and aspartate in B. subtilis. Collectively, our data show how effectively B. subtilis can exploit environmental resources to derive osmotic-stress protection through physiological means.
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