ArticleApplied and environmental microbiology2015
Molecular mechanism of nicotine degradation by a newly isolated strain, Ochrobactrum sp. strain SJY1.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review.Biomolecules · 2023Pooled it
- Ancestral evolution of oxidase activity in a class of (S)-nicotine and (S)-6-hydroxynicotine-degrading flavoenzymes.The Journal of biological chemistry · 2025Article
- Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications.Biotechnology for biofuels and bioproducts · 2024Review
- Effects ofFrontiers in bioengineering and biotechnology · 2024Article
- Bacillus sp. YC7 from intestines of Lasioderma serricorne degrades nicotine due to nicotine dehydrogenase.AMB Express · 2023Article
- PicR as a MarR Family Transcriptional Repressor Multiply Controls the Transcription of Picolinic Acid Degradation Gene ClusterApplied and environmental microbiology · 2022Article
- Nicotine metabolism pathway in bacteria: mechanism, modification, and application.Applied microbiology and biotechnology · 2022Review
- Hexabromocyclododecanes Are Dehalogenated by CYP168A1 fromApplied and environmental microbiology · 2021Article
- Rid Enhances the 6-Hydroxypseudooxynicotine Dehydrogenase Reaction in Nicotine Degradation by Agrobacterium tumefaciens S33.Applied and environmental microbiology · 2021Article
- Additional Role of Nicotinic Acid Hydroxylase for the Transformation of 3-Succinoyl-Pyridine byApplied and environmental microbiology · 2021Article
- Differential Effects of Homologous Transcriptional Regulators NicR2A, NicR2B1, and NicR2B2 and Endogenous Ectopic Strong Promoters on Nicotine Metabolism inApplied and environmental microbiology · 2021Article
- Direct Evidence That Sunbirds' Gut Microbiota Degrades Floral Nectar's Toxic Alkaloids.Frontiers in microbiology · 2021Article
- Structural Insights into 6-Hydroxypseudooxynicotine Amine Oxidase fromApplied and environmental microbiology · 2020Article
- Physiology of a Hybrid Pathway for Nicotine Catabolism in Bacteria.Frontiers in microbiology · 2020Review
- Identification and Characterization of a NovelJournal of bacteriology · 2019Article
- 6-Hydroxypseudooxynicotine Dehydrogenase Delivers Electrons to Electron Transfer Flavoprotein during Nicotine Degradation by Agrobacterium tumefaciens S33.Applied and environmental microbiology · 2019Article
- Novel 3,6-Dihydroxypicolinic Acid Decarboxylase-Mediated Picolinic Acid Catabolism inJournal of bacteriology · 2019Article
- Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822.BMC genomics · 2019Article
- ndpT encodes a new protein involved in nicotine catabolism by Sphingomonas melonis TY.Applied microbiology and biotechnology · 2018Article
- A Novel Degradation Mechanism for Pyridine Derivatives in Alcaligenes faecalis JQ135.Applied and environmental microbiology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A newly isolated strain, SJY1, identified as Ochrobactrum sp., utilizes nicotine as a sole source of carbon, nitrogen, and energy. Strain SJY1 could efficiently degrade nicotine via a variant of the pyridine and pyrrolidine pathways (the VPP pathway), which highlights bacterial metabolic diversity in relation to nicotine degradation. A 97-kbp DNA fragment containing six nicotine degradation-related genes was obtained by gap closing from the genome sequence of strain SJY1. Three genes, designated vppB, vppD, and vppE, in the VPP pathway were cloned and heterologously expressed, and the related proteins were characterized. The vppB gene encodes a flavin-containing amine oxidase converting 6-hydroxynicotine to 6-hydroxy-N-methylmyosmine. Although VppB specifically catalyzes the dehydrogenation of 6-hydroxynicotine rather than nicotine, it shares higher amino acid sequence identity with nicotine oxidase (38%) from the pyrrolidine pathway than with its isoenzyme (6-hydroxy-l-nicotine oxidase, 24%) from the pyridine pathway. The vppD gene encodes an NADH-dependent flavin-containing monooxygenase, which catalyzes the hydroxylation of 6-hydroxy-3-succinoylpyridine to 2,5-dihydroxypyridine. VppD shows 62% amino acid sequence identity with the hydroxylase (HspB) from Pseudomonas putida strain S16, whereas the specific activity of VppD is ∼10-fold higher than that of HspB. VppE is responsible for the transformation of 2,5-dihydroxypyridine. Sequence alignment and phylogenetic analysis suggested that the VPP pathway, which evolved independently from nicotinic acid degradation, might have a closer relationship with the pyrrolidine pathway. The proteins and functional pathway identified here provide a sound basis for future studies aimed at a better understanding of molecular principles of nicotine degradation.
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