ArticleCancer research2014
Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach.
Article in Cancer research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed.
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- Perspective on the use of fluorescence molecular imaging for peripheral and deepJournal of biomedical optics · 2025Review
- Bioinspired color-near infrared endoscopic imaging system for molecular guided cancer surgery.Journal of biomedical optics · 2023Article
- Identification of a Suitable Untargeted Agent for the Clinical Translation of ABY-029 Paired-Agent Imaging in Fluorescence-Guided Surgery.Molecular imaging and biology · 2023Article
- 3D-Printed Tumor Phantoms for Assessment of In Vivo Fluorescence Imaging Analysis Methods.Molecular imaging and biology · 2023Article
- Paired-agent imaging as a rapidFrontiers in oncology · 2023Article
- Fluorescence molecular optomic signatures improve identification of tumors in head and neck specimens.Frontiers in medical technology · 2023Article
- Examining the Feasibility of Quantifying Receptor Availability Using Cross-Modality Paired-Agent Imaging.Molecular imaging and biology · 2022Article
- Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Multimodal Bone Metastasis-associated Epidermal Growth Factor Receptor Imaging in an Orthotopic Rat Model.Radiology. Imaging cancer · 2021Article
- Is Tumor Cell Specificity Distinct from Tumor Selectivity In Vivo?: A Quantitative NIR Molecular Imaging Analysis of Nanoliposome Targeting.Nano research · 2021Article
- Monitoring cancer cell surface receptor expression during anti-angiogenesis therapy in vivo.Proceedings of SPIE--the International Society for Optical Engineering · 2021Article
- Prediction of optimal contrast times post-imaging agent administration to inform personalized fluorescence-guided surgery.Journal of biomedical optics · 2020Article
- Effect of nonspecific binding of imaging agents to plasma protein in the paired-agent imaging for resection during surgery (PAIRS).Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Estimating drug delivery using hybrid system for simultaneous dynamic MRI and fluorescence tomography.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Developing a novel hyperspectral imaging cryomacrotome for whole body fluorescence imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Estimating paired-agent uptake in altered tumor vasculature using MRI-coupled fluorescence tomography.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Preclinical imaging of epidermal growth factor receptor with ABY-029 in soft-tissue sarcoma for fluorescence-guided surgery and tumor detection.Journal of surgical oncology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
As receptor-targeted therapeutics become increasingly used in clinical oncology, the ability to quantify protein expression and pharmacokinetics in vivo is imperative to ensure successful individualized treatment plans. Current standards for receptor analysis are performed on extracted tissues. These measurements are static and often physiologically irrelevant; therefore, only a partial picture of available receptors for drug targeting in vivo is provided. Until recently, in vivo measurements were limited by the inability to separate delivery, binding, and retention effects, but this can be circumvented by a dual-tracer approach for referencing the detected signal. We hypothesized that in vivo receptor concentration imaging (RCI) would be superior to ex vivo immunohistochemistry (IHC). Using multiple xenograft tumor models with varying EGFR expression, we determined the EGFR concentration in each model using a novel targeted agent (anti-EGFR affibody-IRDye800CW conjugate) along with a simultaneously delivered reference agent (control affibody-IRDye680RD conjugate). The RCI-calculated in vivo receptor concentration was strongly correlated with ex vivo pathologist-scored IHC and computer-quantified ex vivo immunofluorescence. In contrast, no correlation was observed with ex vivo Western blot analysis or in vitro flow-cytometry assays. Overall, our results argue that in vivo RCI provides a robust measure of receptor expression equivalent to ex vivo immunostaining, with implications for use in noninvasive monitoring of therapy or therapeutic guidance during surgery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.