ArticleGenome biology2014
Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially resolved RNA-seq.
Article in Genome biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Spatial Transcriptomic Analysis Reveals Regional Transcript Changes in Early and Late Stages of rd1 Model Mice with Retinitis Pigmentosa.International journal of molecular sciences · 2023Article
- Spatial Reconstruction of Oligo and Single Cells by De Novo Coalescent Embedding of Transcriptomic Networks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Dissecting the brain with spatially resolved multi-omics.Journal of pharmaceutical analysis · 2023Review
- Identification of spatially variable genes with graph cuts.Nature communications · 2022Article
- Review
- Topological data analysis in biomedicine: A review.Journal of biomedical informatics · 2022Review
- Transcriptional heterogeneity of ventricular zone cells in the ganglionic eminences of the mouse forebrain.eLife · 2022Article
- β-Catenin Deletion in Regional Neural Progenitors Leads to Congenital Hydrocephalus in Mice.Neuroscience bulletin · 2022Article
- Spatial tissue profiling by imaging-free molecular tomography.Nature biotechnology · 2021Article
- Tools and Concepts for Interrogating and Defining Cellular Identity.Cell stem cell · 2020Review
- ALK4 coordinates extracellular and intrinsic signals to regulate development of cortical somatostatin interneurons.The Journal of cell biology · 2020Article
- Application of Fused Organoid Models to Study Human Brain Development and Neural Disorders.Frontiers in cellular neuroscience · 2020Review
- Informing disease modelling with brain-relevant functional genomic annotations.Brain : a journal of neurology · 2019Review
- CTCF Governs the Identity and Migration of MGE-Derived Cortical Interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Early emergence of cortical interneuron diversity in the mouse embryo.Science (New York, N.Y.) · 2018Article
- Article
- Cortical interneuron specification: the juncture of genes, time and geometry.Current opinion in neurobiology · 2017Review
- Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons.eLife · 2016Article
- Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling.Nature communications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCortical interneurons originating from the medial ganglionic eminence, MGE, are among the most diverse cells within the CNS. Different pools of proliferating progenitor cells are thought to exist in the ventricular zone of the MGE, but whether the underlying subventricular and mantle regions of the MGE are spatially patterned has not yet been addressed. Here, we combined laser-capture microdissection and multiplex RNA-sequencing to map the transcriptome of MGE cells at a spatial resolution of 50 μm.
resultsDistinct groups of progenitor cells showing different stages of interneuron maturation are identified and topographically mapped based on their genome-wide transcriptional pattern. Although proliferating potential decreased rather abruptly outside the ventricular zone, a ventro-lateral gradient of increasing migratory capacity was identified, revealing heterogeneous cell populations within this neurogenic structure.
conclusionsWe demonstrate that spatially resolved RNA-seq is ideally suited for high resolution topographical mapping of genome-wide gene expression in heterogeneous anatomical structures such as the mammalian central nervous system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.