ArticleDevelopment (Cambridge, England)2014
Quantitative imaging of cell dynamics in mouse embryos using light-sheet microscopy.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 101 citations in OpenAlex.
- Protocol for ultrafast immunolabeling and 3D imaging of whole organs and large tissues.STAR protocols · 2026Article
- High-Fidelity Long-term Whole-embryo Lineage and Fate Reconstruction by Iterative Tracking with Error Correction.bioRxiv : the preprint server for biology · 2026Article
- Discovering the unexpected: Insights into the dynamics of mouse neural tube closure revealed by time-lapse imaging.Developmental biology · 2026Review
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- MIFA: Metadata, Incentives, Formats and Accessibility guidelines to improve the reuse of AI datasets for bioimage analysis.Nature methods · 2025Review
- Emergence of endothelial subtypes and role of cell cycle control in arterial-venous specification during embryonic vascular development.Cell reports · 2025Article
- Quantifying the relationship between cell proliferation and morphology during development of the face.Development (Cambridge, England) · 2025Article
- Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.Human reproduction (Oxford, England) · 2025Article
- Beyond genomic studies of congenital heart defects through systematic modelling and phenotyping.Disease models & mechanisms · 2024Article
- Quantifying DNA damage following light sheet and confocal imaging of the mammalian embryo.Scientific reports · 2024Article
- 3DMOUSEneST: a volumetric label-free imaging method evaluating embryo-uterine interaction and decidualization efficacy.Development (Cambridge, England) · 2024Article
- Viewing early life without labels: optical approaches for imaging the early embryo†.Biology of reproduction · 2024Review
- Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging.Applied optics · 2023Article
- Graded mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart.Cell · 2023Article
- Cell cycle dynamics control fluidity of the developing mouse neuroepithelium.Nature physics · 2023Article
- Imaging the onset of oscillatory signaling dynamics during mouse embryo gastrulation.Development (Cambridge, England) · 2022Article
- An ex vivo system to study cellular dynamics underlying mouse peri-implantation development.Developmental cell · 2022Article
- A light sheet fluorescence microscopy protocol forFrontiers in cell and developmental biology · 2022Article
- Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022Review
- Mouse embryo phenotyping with optical coherence tomography.Frontiers in cell and developmental biology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Single/selective-plane illumination, or light-sheet, systems offer several advantages over other fluorescence microscopy methods for live, 3D microscopy. These systems are valuable for studying embryonic development in several animal systems, such as Drosophila, C. elegans and zebrafish. The geometry of the light path in this form of microscopy requires the sample to be accessible from multiple sides and fixed in place so that it can be rotated around a single axis. Popular methods for mounting include hanging the specimen from a pin or embedding it in 1-2% agarose. These methods can be particularly problematic for certain samples, such as post-implantation mouse embryos, that expand significantly in size and are very delicate and sensitive to mounting. To overcome the current limitations and to establish a robust strategy for long-term (24 h) time-lapse imaging of E6.5-8.5 mouse embryos with light-sheet microscopy, we developed and tested a method using hollow agarose cylinders designed to accommodate for embryonic growth, yet provide boundaries to minimize tissue drift and enable imaging in multiple orientations. Here, we report the first 24-h time-lapse sequences of post-implantation mouse embryo development with light-sheet microscopy. We demonstrate that light-sheet imaging can provide both quantitative data for tracking changes in morphogenesis and reveal new insights into mouse embryogenesis. Although we have used this approach for imaging mouse embryos, it can be extended to imaging other types of embryos as well as tissue explants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.