ArticleACS chemical biology2015
Fluorogen-activating proteins provide tunable labeling densities for tracking FcεRI independent of IgE.
Article in ACS chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- IgE Occupancy and Antigen Valency Cooperate to Control FcεRI Aggregation Geometry and Signaling Efficiency.International journal of molecular sciences · 2026Article
- IgE occupancy and antigen valency cooperate to control FcεRI aggregation geometry and signaling efficiency.bioRxiv : the preprint server for biology · 2025Article
- Antigen properties shape organization of FcεRI aggregates to tune mast cell signaling.bioRxiv : the preprint server for biology · 2025Article
- Fluorescent labeling of proteins in vitro and in vivo using encoded peptide tags.The Journal of biological chemistry · 2025Review
- Genetically Encoded Activators of Small Molecules for Imaging and Drug Delivery.Angewandte Chemie (International ed. in English) · 2020Review
- Precisely calibrated and spatially informed illumination for conventional fluorescence and improved PALM imaging applications.Methods and applications in fluorescence · 2020Article
- Covalently Tethered Rhodamine Voltage Reporters for High Speed Functional Imaging in Brain Tissue.Journal of the American Chemical Society · 2020Article
- Combinatorial diversity of Syk recruitment driven by its multivalent engagement with FcεRIγ.Molecular biology of the cell · 2019Article
- Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System.ACS chemical biology · 2019Article
- Antibody-Linked Fluorogen-Activating Proteins for Antigen Detection and Cell Ablation.Bioconjugate chemistry · 2019Article
- Mechanisms of inside-out signaling of the high-affinity IgG receptor FcγRI.Science signaling · 2018Article
- Fluorogen-activating proteins: beyond classical fluorescent proteins.Acta pharmaceutica Sinica. B · 2018Review
- Article
- Differential mast cell outcomes are sensitive to FcεRI-Syk binding kinetics.Molecular biology of the cell · 2017Article
- Fluorogenic Labeling Strategies for Biological Imaging.International journal of molecular sciences · 2017Review
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule.Journal of the American Chemical Society · 2016Article
- Tetraspanin CD82 Regulates the Spatiotemporal Dynamics of PKCα in Acute Myeloid Leukemia.Scientific reports · 2016Article
- Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Enhanced dimerization drives ligand-independent activity of mutant epidermal growth factor receptor in lung cancer.Molecular biology of the cell · 2015Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Crosslinking of IgE bound FcεRI on mast cells and basophils by multivalent antigen leads to degranulation and the release of key inflammatory mediators that stimulate the allergic response. Here, we present and characterize the use of fluorogen-activating proteins (FAPs) for single particle tracking of FcεRI to investigate how receptor mobility is influenced after IgE-induced changes in mast cell behavior. FAPs are genetically encoded tags that bind a fluorogen dye and increase its brightness upon binding up to 20,000-fold. We demonstrate that, by titrating fluorogen concentration, labeling densities from ensemble to single particle can be achieved, independent of expression level and without the need for wash steps or photobleaching. The FcεRI γ-subunit fused to a FAP (FAP-γ) provides, for the first time, an IgE-independent probe for tracking this signaling subunit of FcεRI at the single molecule level. We show that the FcεRI γ-subunit dynamics are controlled by the IgE-binding α-subunit and that the cytokinergic IgE, SPE-7, induces mast cell activation without altering FcεRI mobility or promoting internalization. We take advantage of the far-red emission of the malachite green (MG) fluorogen to track FcεRI relative to dynamin-GFP and find that immobilized receptors readily correlate with locations of dynamin recruitment only under conditions that promote rapid endocytosis. These studies demonstrate the usefulness of the FAP system for single molecule studies and have provided new insights into the relationship among FcεRI structure, activity, and mobility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.