ArticlePloS one2014
Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- TBX3 is essential for establishment of the posterior boundary of anterior genes and upregulation of posterior genes together with HAND2 during the onset of limb bud development.Development (Cambridge, England) · 2024Article
- Validation of a new Custom Polyclonal Progesterone Receptor Antibody for Immunohistochemistry in the Female Mouse Brain.Journal of the Endocrine Society · 2023Article
- TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration.BMC biology · 2023Article
- The Role of CEP55 Expression in Tumor Immune Response and Prognosis of Patients with Non-small Cell lung Cancer.Archives of Iranian medicine · 2022Article
- Estrogen receptor-α signaling in post-natal mammary development and breast cancers.Cellular and molecular life sciences : CMLS · 2021Review
- Overexpression of TBX3 transcription factor as a potential diagnostic marker for breast cancer.Molecular and clinical oncology · 2019Article
- Hypogonadotropic hypogonadism and pituitary hypoplasia as recurrent features in Ulnar-Mammary syndrome.Endocrine connections · 2018Article
- Serially transplantable mammary epithelial cells express the Thy-1 antigen.Breast cancer research : BCR · 2018Article
- Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals.Biology open · 2018Article
- Genetic variation in sensitivity to estrogens and breast cancer risk.Mammalian genome : official journal of the International Mammalian Genome Society · 2018Review
- A Dynamic Role of TBX3 in the Pluripotency Circuitry.Stem cell reports · 2015Article
- Hormone-sensing mammary epithelial progenitors: emerging identity and hormonal regulation.Journal of mammary gland biology and neoplasia · 2015Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional repressor Tbx3 is involved in lineage specification in several tissues during embryonic development. Germ-line mutations in the Tbx3 gene give rise to Ulnar-Mammary Syndrome (comprising reduced breast development) and Tbx3 is required for mammary epithelial cell identity in the embryo. Notably Tbx3 has been implicated in breast cancer, which develops in adult mammary epithelium, but the role of Tbx3 in distinct cell types of the adult mammary gland has not yet been characterized. Using a fluorescent reporter knock-in mouse, we show that in adult virgin mice Tbx3 is highly expressed in luminal cells that express hormone receptors, and not in luminal cells of the alveolar lineage (cells primed for milk production). Flow cytometry identified Tbx3 expression already in progenitor cells of the hormone-sensing lineage and co-immunofluorescence confirmed a strict correlation between estrogen receptor (ER) and Tbx3 expression in situ. Using in vivo reconstitution assays we demonstrate that Tbx3 is functionally relevant for this lineage because knockdown of Tbx3 in primary mammary epithelial cells prevented the formation of ER+ cells, but not luminal ER- or basal cells. Interestingly, genes that are repressed by Tbx3 in other cell types, such as E-cadherin, are not repressed in hormone-sensing cells, highlighting that transcriptional targets of Tbx3 are cell type specific. In summary, we provide the first analysis of Tbx3 expression in the adult mammary gland at a single cell level and show that Tbx3 is important for the generation of hormone-sensing cells.
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