ArticleScientific reports2014
In vitro nanobody discovery for integral membrane protein targets.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Design and validation of a ribosome display library for synthetic nanobody selection.Advanced biotechnology · 2026Article
- Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nanobodies: From Discovery to AI-Driven Design.Biology · 2025Review
- Rapid discovery and evolution of nanosensors containing fluorogenic amino acids.Nature communications · 2024Article
- mRNA Display Pipeline for Protein Biosensor Construction.ACS sensors · 2024Article
- Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications.Biosensors · 2024Review
- A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies.Frontiers in bioengineering and biotechnology · 2022Article
- Improved Methods for Single-Molecule Fluorescence In Situ Hybridization and Immunofluorescence in Caenorhabditis elegans Embryos.Current protocols · 2021Article
- Directing evolution of novel ligands by mRNA display.Chemical Society reviews · 2021Review
- Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique.The Journal of biological chemistry · 2021Review
- Discovery of Antivirals Using Phage Display.Viruses · 2021Review
- Nanobodies targeting immune checkpoint molecules for tumor immunotherapy and immunoimaging (Review).International journal of molecular medicine · 2021Review
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Exploring cellular biochemistry with nanobodies.The Journal of biological chemistry · 2020Review
- Enabling Flow-Based Kinetic Off-Rate Selections Using a Microfluidic Enrichment Device.Analytical chemistry · 2020Article
- Identification and characterization of adipose surface epitopes.The Biochemical journal · 2020Review
- Evaluation of the yeast surface display system for screening of functional nanobodies.AMB Express · 2020Article
- Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.Methods in cell biology · 2019Review
- Synthetic single domain antibodies for the conformational trapping of membrane proteins.eLife · 2018Article
- A Single Domain-Based Anti-Her2 Antibody Has Potent Antitumor Activities.Translational oncology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose transporter, GLUT-1. We envision our streamlined method as a bench-top platform technology, in combination with various molecular evolution techniques, for expedited Nb discovery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.