ArticleCold Spring Harbor protocols2014
An optimized protocol for high-throughput in situ hybridization of zebra finch brain.
Article in Cold Spring Harbor protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Resilience of A Learned Motor Behavior After Chronic Disruption of Inhibitory Circuits.bioRxiv : the preprint server for biology · 2024Article
- Genomic loss of GPR108 disrupts AAV transduction in birds.bioRxiv : the preprint server for biology · 2024Article
- Cell type specializations of the vocal-motor cortex in songbirds.Cell reports · 2023Article
- Bats possess the anatomical substrate for a laryngeal motor cortex.bioRxiv : the preprint server for biology · 2023Article
- Motor cortex analogue neurons in songbirds utilize Kv3 channels to generate ultranarrow spikes.eLife · 2023Article
- Article
- Light-induced shifts in opsin gene expression in the four-eyed fishFrontiers in neuroscience · 2022Article
- Resurgent NaNature communications · 2021Article
- Molecular specializations of deep cortical layer analogs in songbirds.Scientific reports · 2020Article
- Acute social isolation alters neurogenomic state in songbird forebrain.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- ZEBrA: Zebra finch Expression Brain Atlas-A resource for comparative molecular neuroanatomy and brain evolution studies.The Journal of comparative neurology · 2020Article
- Article
- Molecular architecture of the zebra finch arcopallium.The Journal of comparative neurology · 2019Article
- Exploring the molecular basis of neuronal excitability in a vocal learner.BMC genomics · 2019Article
- Seasonal changes in the song control nuclei of the Rufous-bellied Thrush, Turdus rufiventris (Oscine, Passeriformes, and Turdidae).Journal of experimental zoology. Part B, Molecular and developmental evolution · 2019Article
- Urotensin-related gene transcripts mark developmental emergence of the male forebrain vocal control system in songbirds.Scientific reports · 2019Article
- The constitutive differential transcriptome of a brain circuit for vocal learning.BMC genomics · 2018Article
- Dynamic gene expression in the song system of zebra finches during the song learning period.Developmental neurobiology · 2015Article
- Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles).Scientific reports · 2015Article
- The zebra finch, Taeniopygia guttata: an avian model for investigating the neurobiological basis of vocal learning.Cold Spring Harbor protocols · 2014Review
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6 authors.
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Abstract
In situ hybridization (ISH) is a sensitive technique for documenting the tissue distribution of mRNAs. Advanced nonradioactive ISH methods that are based on the use of digoxigenin (DIG)-labeled probes and chromogenic detection have better spatial resolution than emulsion autoradiography techniques and, when paired with high-resolution digital imaging, allow for large-scale profiling of gene expression at cellular resolution within a histological context. However, technical challenges restrict the number of genes that can be investigated in a small laboratory setting. This protocol describes an optimized, low-cost, small-footprint, high-throughput ISH procedure to detect gene expression patterns in 10-µm brain sections from zebra finches. It uses DIG-labeled riboprobes synthesized from cDNA templates available through the Songbird Neurogenomics Consortium. The method is compatible with high-resolution digital imaging; it produces images with low background and a resolution approaching that of immunohistochemical methods. Approximately 180 slides can be processed each week using this protocol, but it can be scaled to accommodate a broad range of tissues from which cryosections can be obtained.
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