Evidence map›Paper›PMID 25341867›Full record

ArticlePlant molecular biology2015

De novo sequencing and analysis of the lily pollen transcriptome: an open access data source for an orphan plant species.

Veronika Lang, Björn Usadel, Gerhard Obermeyer

Abstract read
PubMed Publisher
In one paragraph

Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Identification of a Male Sterile Candidate Gene inFrontiers in plant science · 2022
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Bioinformatics resources for pollen.Plant reproduction · 2016
    Review
  10. Article
  11. Article
  12. A decade of pollen transcriptomics.Plant reproduction · 2015
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Veronika LangMolecular Plant Biophysics and Biochemistry, Depatment of Molecular Biology, University of Salzburg, Billrothstr.11, 5020, Salzburg, Austria.
Björn Usadel
Gerhard Obermeyer

Funding

Austrian Science Fund FWF P 21298
6 · The paper itself

Abstract

Pollen grains of Lilium longiflorum are a long-established model system for pollen germination and tube tip growth. Due to their size, protein content and almost synchronous germination in synthetic media, they provide a simple system for physiological measurements as well as sufficient material for biochemical studies like protein purifications, enzyme assays, organelle isolation or determination of metabolites during germination and pollen tube elongation. Despite recent progresses in molecular biology techniques, sequence information of expressed proteins or transcripts in lily pollen is still scarce. Using a next generation sequencing strategy (RNAseq), the lily pollen transcriptome was investigated resulting in more than 50 million high quality reads with a length of 90 base pairs. Sequenced transcripts were assembled and annotated, and finally visualized with MAPMAN software tools and compared with other RNAseq or genome data including Arabidopsis pollen, Lilium vegetative tissues and the Amborella trichopoda genome. All lily pollen sequence data are provided as open access files with suitable tools to search sequences of interest.

Indexed as

Transcriptome14-3-3 ProteinsGenes, PlantLiliumPhylogenyPlant ProteinsPollenSequence Analysis, RNA14-3-3 ProteinsPlant Proteins

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.