ArticleJournal of biomedical optics2014
Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier.
Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Photochemical Internalization of Etoposide Using Dendrimer Nanospheres Loaded with Etoposide and Protoporphyrin IX on a Glioblastoma Cell Line.Pharmaceutics · 2021Article
- Photochemical Internalization for Intracellular Drug Delivery. From Basic Mechanisms to Clinical Research.Journal of clinical medicine · 2020Review
- Photochemical Internalization Enhanced Nonviral Suicide Gene Therapy.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens.Nucleic acids research · 2018Article
- Non-viral Delivery of Nucleic Acids: Insight Into Mechanisms of Overcoming Intracellular Barriers.Frontiers in pharmacology · 2018Review
- Photodynamic therapy enhances the efficacy of gene-directed enzyme prodrug therapy.Photodiagnosis and photodynamic therapy · 2017Article
- Trigger-Responsive Gene Transporters for Anticancer Therapy.Nanomaterials (Basel, Switzerland) · 2017Review
- Synergistic efficacy of ultrasound, sonosensitizers and chemotherapy: a review.Therapeutic delivery · 2017Review
- Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment.Beilstein journal of nanotechnology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either “naked” polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS 2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.