Evidence map›Paper›PMID 25340824›Full record

ArticlePloS one2014

Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics.

Ryusei Tanaka, Akina Hino, Isheng J Tsai, Juan Emilio Palomares-Rius, Ayako Yoshida, Yoshitoshi Ogura, Tetsuya Hayashi, Haruhiko Maruyama, Taisei Kikuchi

Abstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
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  12. Detection of zoonoticParasite epidemiology and control · 2024
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  13. Article
  14. Parasite effects on receivers in animal communication: Hidden impacts on behavior, ecology, and evolution.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  15. Article
  16. Article
  17. Investigation of Parasitic Infection in Crocodile Lizards (Animals : an open access journal from MDPI · 2022
    Article
  18. Review
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  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ryusei TanakaDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Akina HinoDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Isheng J TsaiBiodiversity Research Center, Academia Sinica, Taipei, Taiwan.
Juan Emilio Palomares-RiusDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Ayako YoshidaDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Yoshitoshi OguraDivision of Microbial Genomics, Department of Genomics and Bioenviromental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
Tetsuya HayashiDivision of Microbial Genomics, Department of Genomics and Bioenviromental Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan.
Haruhiko MaruyamaDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Taisei KikuchiDivision of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis has been restricted because of the difficult, highly specialised and time-consuming processes involved in parasite identification. In this study, we assessed parasite diversity in wild rats using 18S rDNA-based metagenomics. 18S rDNA PCR products were sequenced using an Illumina MiSeq sequencer and the analysis of the sequences using the QIIME software successfully classified them into several parasite groups. The comparison of the results with those obtained using standard methods including microscopic observation of helminth parasites in the rat intestines and PCR amplification/sequencing of 18S rDNA from isolated single worms suggests that this new technique is reliable and useful to investigate parasite diversity.

Indexed as

BiodiversityMetagenomicsAnimalsAnimals, WildDatabases, GeneticHelminthsIntestinesParasitesPhylogenyRatsRNA, Ribosomal, 18SSequence Analysis, DNASoftwareSpecies SpecificityRNA, Ribosomal, 18S

Identifiers

PMID25340824
PMCPMC4207705

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.