ArticlePLoS genetics2014
The master activator of IncA/C conjugative plasmids stimulates genomic islands and multidrug resistance dissemination.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 129 citations in OpenAlex.
- Conjugative transfer inhibition of IncA and IncC plasmids by SGI1-like elements via relaxosome assembly interference.Nucleic acids research · 2026Article
- Ecological theory sheds light on plasmid diversity and dynamics.PLoS biology · 2026Article
- Genetic Elements Associated with the Acquired Resistome of the Gut Microbiota in a Broiler Rooster Flock in Hungary.Animals : an open access journal from MDPI · 2026Article
- Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli.Nature communications · 2025Article
- Interactions and evolutionary relationships among bacterial mobile genetic elements.Nature reviews. Microbiology · 2025Review
- Mechanisms of Transmission and Adaptation ofVeterinary sciences · 2025Article
- Article
- Mobile genetic elements encoding antibiotic resistance genes and virulence genes inFrontiers in microbiology · 2025Review
- Genomic evidence of two-staged transmission of the early seventh cholera pandemic.Nature communications · 2024Article
- Surface exclusion of IncC conjugative plasmids and their relatives.PLoS genetics · 2024Article
- Hijackers, hitchhikers, or co-drivers? The mysteries of mobilizable genetic elements.PLoS biology · 2024Article
- Multidrug resistance inApplied and environmental microbiology · 2024Article
- Two birds with one stone: SGI1 can stabilize itself and expel the IncC helper by hijacking the plasmid parABS system.Nucleic acids research · 2024Article
- A comprehensive list of genes required for the efficient conjugation of plasmid Rts1 was determined by systematic deletion analysis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024Article
- Host range of strand-biased circularizing integrative elements: a new class of mobile DNA elements nesting in Gammaproteobacteria.Mobile DNA · 2023Article
- Conjugative IncC Plasmid Entry Triggers the SOS Response and Promotes Effective Transfer of the Integrative Antibiotic Resistance Element SGI1.Microbiology spectrum · 2023Article
- Article
- Overview ofFrontiers in microbiology · 2022Article
- Article
- Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.Molecular microbiology · 2021Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dissemination of antibiotic resistance genes occurs mostly by conjugation, which mediates DNA transfer between cells in direct contact. Conjugative plasmids of the IncA/C incompatibility group have become a substantial threat due to their broad host-range, the extended spectrum of antimicrobial resistance they confer, their prevalence in enteric bacteria and their very efficient spread by conjugation. However, their biology remains largely unexplored. Using the IncA/C conjugative plasmid pVCR94ΔX as a prototype, we have investigated the regulatory circuitry that governs IncA/C plasmids dissemination and found that the transcriptional activator complex AcaCD is essential for the expression of plasmid transfer genes. Using chromatin immunoprecipitation coupled with exonuclease digestion (ChIP-exo) and RNA sequencing (RNA-seq) approaches, we have identified the sequences recognized by AcaCD and characterized the AcaCD regulon. Data mining using the DNA motif recognized by AcaCD revealed potential AcaCD-binding sites upstream of genes involved in the intracellular mobility functions (recombination directionality factor and mobilization genes) in two widespread classes of genomic islands (GIs) phylogenetically unrelated to IncA/C plasmids. The first class, SGI1, confers and propagates multidrug resistance in Salmonella enterica and Proteus mirabilis, whereas MGIVmi1 in Vibrio mimicus belongs to a previously uncharacterized class of GIs. We have demonstrated that through expression of AcaCD, IncA/C plasmids specifically trigger the excision and mobilization of the GIs at high frequencies. This study provides new evidence of the considerable impact of IncA/C plasmids on bacterial genome plasticity through their own mobility and the mobilization of genomic islands.
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