ArticlePLoS genetics2014
A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- Expression of conjugation genes is controlled by a novel zipper-type transcriptional attenuation mechanism.Nucleic acids research · 2026Article
- Genetic Engineering of Bacillus subtilis Using Competence-Induced Homologous Recombination Techniques.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Extraordinary long-stem confers resistance of intrinsic terminators to processive antitermination.Nucleic acids research · 2023Article
- A tetramerization domain in prokaryotic and eukaryotic transcription regulators homologous to p53.Acta crystallographica. Section D, Structural biology · 2023Article
- pLS20 is the archetype of a new family of conjugative plasmids harboured byNAR genomics and bioinformatics · 2021Article
- Assessment ofMicroorganisms · 2021Article
- A novel bipartite antitermination system widespread in conjugative elements of Gram-positive bacteria.Nucleic acids research · 2021Article
- A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13.Communications biology · 2021Article
- Article
- Multiple Layered Control of the Conjugation Process of theFrontiers in molecular biosciences · 2021Review
- Reversible regulation of conjugation of Bacillus subtilis plasmid pLS20 by the quorum sensing peptide responsive anti-repressor RappLS20.Nucleic acids research · 2020Article
- Inactivation of the dimeric RappLS20 anti-repressor of the conjugation operon is mediated by peptide-induced tetramerization.Nucleic acids research · 2020Article
- Unravelling the γ-butyrolactone network in Streptomyces coelicolor by computational ensemble modelling.PLoS computational biology · 2020Article
- Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System ofFrontiers in microbiology · 2019Article
- Regulation of Gram-Positive Conjugation.Frontiers in microbiology · 2019Review
- A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus.Microbial cell factories · 2018Article
- Cell fate potentials and switching kinetics uncovered in a classic bistable genetic switch.Nature communications · 2018Article
- Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer.Microbial cell factories · 2018Article
- Mechanisms of peptide sex pheromone regulation of conjugation in Enterococcus faecalis.MicrobiologyOpen · 2017Article
- Discovery of a new family of relaxases in Firmicutes bacteria.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features. Little is known about regulation of conjugation systems present on plasmids from Gram-positive bacteria. pLS20 is a native conjugative plasmid from the Gram-positive bacterium Bacillus subtilis. Recently the key players that repress and activate pLS20 conjugation have been identified. Here we studied in detail the molecular mechanism regulating the pLS20 conjugation genes using both in vivo and in vitro approaches. Our results show that conjugation is subject to the control of a complex genetic switch where at least three levels of regulation are integrated. The first of the three layers involves overlapping divergent promoters of different strengths regulating expression of the conjugation genes and the key transcriptional regulator RcoLS20. The second layer involves a triple function of RcoLS20 being a repressor of the main conjugation promoter and an activator and repressor of its own promoter at low and high concentrations, respectively. The third level of regulation concerns formation of a DNA loop mediated by simultaneous binding of tetrameric RcoLS20 to two operators, one of which overlaps with the divergent promoters. The combination of these three layers of regulation in the same switch allows the main conjugation promoter to be tightly repressed during conditions unfavorable to conjugation while maintaining the sensitivity to accurately switch on the conjugation genes when appropriate conditions occur. The implications of the regulatory switch and comparison with other genetic switches involving DNA looping are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.