ArticlePloS one2014
A murine inhalation model to characterize pulmonary exposure to dry Aspergillus fumigatus conidia.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Establishment of a novel murine model of allergic bronchopulmonary aspergillosis using an eosinophilic mutant mouse.The Journal of veterinary medical science · 2026Article
- Loss of pulmonary tissue protection and neutrophil microbicidal defects promote severeInfection and immunity · 2025Article
- Impact of CD11cFrontiers in fungal biology · 2025Article
- Adjuvant-independent airway sensitization and infection mouse models leading to allergic asthma.Frontiers in allergy · 2024Review
- Optimization ofJournal of fungi (Basel, Switzerland) · 2023Article
- Strategies for Monitoring Microbial Life in Beach Sand for Protection of Public Health.International journal of environmental research and public health · 2023Review
- PersistingFrontiers in cellular and infection microbiology · 2023Article
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- Inhalation ofAmerican journal of respiratory cell and molecular biology · 2020Article
- Ten questions concerning the implications of carpet on indoor chemistry and microbiology.Building and environment · 2019Article
- Animal Models to Study Mucormycosis.Journal of fungi (Basel, Switzerland) · 2019Review
- Aspergillus fumigatus viability drives allergic responses to inhaled conidia.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2018Article
- Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis.BMC microbiology · 2018Article
- MicroRNA Regulation of Host Immune Responses following Fungal Exposure.Frontiers in immunology · 2018Review
- Rodent Models of Invasive Aspergillosis due toFrontiers in microbiology · 2017Review
- Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic inhalation exposure.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2016Article
- Subchronic exposures to fungal bioaerosols promotes allergic pulmonary inflammation in naïve mice.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2016Article
- Allergic Inflammation in Aspergillus fumigatus-Induced Fungal Asthma.Current allergy and asthma reports · 2015Review
- Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Most murine models of fungal exposure are based on the delivery of uncharacterized extracts or liquid conidia suspensions using aspiration or intranasal approaches. Studies that model exposure to dry fungal aerosols using whole body inhalation have only recently been described. In this study, we aimed to characterize pulmonary immune responses following repeated inhalation of conidia utilizing an acoustical generator to deliver dry fungal aerosols to mice housed in a nose only exposure chamber. Immunocompetent female BALB/cJ mice were exposed to conidia derived from Aspergillus fumigatus wild-type (WT) or a melanin-deficient (Δalb1) strain. Conidia were aerosolized and delivered to mice at an estimated deposition dose of 1×105 twice a week for 4 weeks (8 total). Histopathological and immunological endpoints were assessed 4, 24, 48, and 72 hours after the final exposure. Histopathological analysis showed that conidia derived from both strains induced lung inflammation, especially at 24 and 48 hour time points. Immunological endpoints evaluated in bronchoalveolar lavage fluid (BALF) and the mediastinal lymph nodes showed that exposure to WT conidia led to elevated numbers of macrophages, granulocytes, and lymphocytes. Importantly, CD8+ IL17+ (Tc17) cells were significantly higher in BALF and positively correlated with germination of A. fumigatus WT spores. Germination was associated with specific IgG to intracellular proteins while Δalb1 spores elicited antibodies to cell wall hydrophobin. These data suggest that inhalation exposures may provide a more representative analysis of immune responses following exposures to environmentally and occupationally prevalent fungal contaminants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.