ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2014
Effects of chronic kidney disease on liver transport: quantitative intravital microscopy of fluorescein transport in the rat liver.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Effect of Roxadustat on the Pharmacokinetics of Simvastatin, Rosuvastatin, and Atorvastatin in Healthy Subjects: Results From 3 Phase 1, Open-Label, 1-Sequence, Crossover Studies.Clinical pharmacology in drug development · 2022Trial
- Evaluation of Blood-CSF Barrier Transport by Quantitative Real Time Fluorescence Microscopy.Pharmaceutical research · 2022Article
- Mitochondrial depolarization and repolarization in the early stages of acetaminophen hepatotoxicity in mice.Toxicology · 2020Article
- Quantitative Kinetic Models from Intravital Microscopy: A Case Study Using Hepatic Transport.The journal of physical chemistry. B · 2019Article
- Drug Transporters in Xenobiotic Disposition and Pharmacokinetic Prediction.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- Indoxyl Sulfate Upregulates Liver P-Glycoprotein Expression and Activity through Aryl Hydrocarbon Receptor Signaling.Journal of the American Society of Nephrology : JASN · 2018Article
- Article
- Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.Methods (San Diego, Calif.) · 2017Article
- Intravital microscopy of biosensor activities and intrinsic metabolic states.Methods (San Diego, Calif.) · 2017Article
- Imaging of anticancer drug action in single cells.Nature reviews. Cancer · 2017Review
- A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy.Nature protocols · 2016Article
- A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy.American journal of physiology. Cell physiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Clinical studies indicate that hepatic drug transport may be altered in chronic kidney disease (CKD). Uremic solutes associated with CKD have been found to alter the expression and/or activity of hepatocyte transporters in experimental animals and in cultured cells. However, given the complexity and adaptability of hepatic transport, it is not clear whether these changes translate into significant alterations in hepatic transport in vivo. To directly measure the effect of CKD on hepatocyte transport in vivo, we conducted quantitative intravital microscopy of transport of the fluorescent organic anion fluorescein in the livers of rats following 5/6th nephrectomy, an established model of CKD. Our quantitative analysis of fluorescein transport showed that the rate of hepatocyte uptake was reduced by ∼20% in 5/6th nephrectomized rats, consistent with previous observations of Oatp downregulation. However, the overall rate of transport into bile canaliculi was unaffected, suggesting compensatory changes in Mrp2-mediated secretion. Our study suggests that uremia resulting from 5/6th nephrectomy does not significantly impact the overall hepatic clearance of an Oatp substrate.
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Registered trials
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