ArticleAngewandte Chemie (International ed. in English)2014
Enzymatic fabrication of high-density RNA arrays.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Enzymatic Synthesis of High-Density RNA Microarrays.Current protocols · 2023Article
- Simple synthesis of massively parallel RNA microarrays via enzymatic conversion from DNA microarrays.Nature communications · 2022Article
- Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.Chemical reviews · 2020Article
- l-DNA Duplex Formation as a Bioorthogonal Information Channel in Nucleic Acid-Based Surface Patterning.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Generation of small molecule-binding RNA arrays and their application to fluorogen-binding RNA aptamers.Methods (San Diego, Calif.) · 2019Article
- Multi-level patterning nucleic acid photolithography.Nature communications · 2019Article
- Encrypted Oligonucleotide Arrays for Molecular Authentication.ACS combinatorial science · 2019Article
- Spotting, Transcription andComputational and structural biotechnology journal · 2019Review
- High-Density RNA Microarrays Synthesized In Situ by Photolithography.Angewandte Chemie (International ed. in English) · 2018Article
- High-Efficiency Reverse (5'→3') Synthesis of Complex DNA Microarrays.Scientific reports · 2018Article
- High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions.Nucleic acids research · 2018Article
- Parallel DNA Synthesis on Poly(ethylene terephthalate).Chembiochem : a European journal of chemical biology · 2017Article
- Mapping the affinity landscape of Thrombin-binding aptamers on 2΄F-ANA/DNA chimeric G-Quadruplex microarrays.Nucleic acids research · 2017Article
- High-Power 365 nm UV LED Mercury Arc Lamp Replacement for Photochemistry and Chemical Photolithography.ACS sustainable chemistry & engineering · 2017Article
- Express photolithographic DNA microarray synthesis with optimized chemistry and high-efficiency photolabile groups.Journal of nanobiotechnology · 2016Article
- Photolithographic Synthesis of High-Density DNA and RNA Arrays on Flexible, Transparent, and Easily Subdivided Plastic Substrates.Analytical chemistry · 2015Article
- Next-Generation o-Nitrobenzyl Photolabile Groups for Light-Directed Chemistry and Microarray Synthesis.Angewandte Chemie (International ed. in English) · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A powerful new strategy for the fabrication of high-density RNA arrays is described. A high-density DNA array is fabricated by standard photolithographic methods, the surface-bound DNA molecules are enzymatically copied into their RNA complements from a surface-bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behind the desired RNA array. The strategy is compatible with 2'-fluoro-modified (2'F) ribonucleoside triphosphates (rNTPs), which may be included in the polymerase extension reaction to impart nuclease resistance and other desirable characteristics to the synthesized RNAs. The use and fidelity of the arrays are explored with DNA hybridization, DNAzyme cleavage, and nuclease digestion experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.