Evidence map›Paper›PMID 25339497›Full record

ArticleMolecular medicine reports2015

Functional expression, characterization and application of the human S100A4 protein.

Degang Wang, Jianwei Zhang, Ziquan Liu, Yunyun Chen, Chuanxiang Xu, Zhiqing Zhang, Xiaohua Liu, Lei Wu, Xuesi Zhou, Xiangyan Meng and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Degang WangPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Jianwei ZhangPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Ziquan LiuPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Yunyun ChenPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Chuanxiang XuPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Zhiqing ZhangPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Xiaohua LiuPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Lei WuPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Xuesi ZhouPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Xiangyan MengPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Hua LiEndoscopy Division, Tianjin Medical University Cancer Hospital and City Key Laboratory of Tianjin Cancer Center, Tianjin 300060, P.R. China.
Hongtao LiuPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Zifeng JiangDivision of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Chaoyang, Beijing 100101, P.R. China.
Tianhui WangPerformance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China.
Chinese Academy of Sciences · CNTianjin Medical University Cancer Institute and Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Preparations utilizing monoclonal antibodies against S100A4 provide useful tools for functional studies to investigate the clinical applications of the human S100A4 protein. In the present study, human S100A4 protein was expressed in Escherichia coli (E. coli) BL21 (DE3), successfully purified by diethylaminoethyl cellulose anion-exchange chromatography and identified by western blot analysis. Soluble S100A4 bioactivity was confirmed by Transwell migration and invasion assays in the human HeLa cell line. Monoclonal antibodies (mAbs) were generated utilizing the standard hybridoma method and were validated by enzyme-linked immunosorbent assay and western blot analysis. The antibody was then used to examine human gastric carcinoma specimens by immunohistochemistry. Recombinant S100A4 was functionally expressed in E. coli and promoted the migration and invasion of HeLa cells. Four hybridoma cell lines, which secreted mAbs specifically against human S100A4 protein, were obtained. One of the four mAbs, namely 2A12D10B2, recognized human S100A4 as indicated by immunohistochemical staining of human gastric carcinoma specimens and recombinant S100A4 was functionally expressed in E. coli. The mAbs of recombinant S100A4 were suitable for detecting S100A4 expression in human tissues and for investigating the subsequent clinical applications of the protein.

Indexed as

HumansS100 Calcium-Binding Protein A4S100 ProteinsS100A4 protein, humanS100 Calcium-Binding Protein A4S100 Proteins

Identifiers

PMID25339497
PMCPMC4237081
OpenAlexW1979599961

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.