ArticleJournal of clinical microbiology2015
Development of an efficient entire-capsid-coding-region amplification method for direct detection of poliovirus from stool extracts.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- An outbreak of atypical hand, foot and mouth disease associated Coxsackievirus A6 in children from Cape Verde, 2023.Virology journal · 2025Article
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- Near-source passive sampling for monitoring viral outbreaks within a university residential setting.Epidemiology and infection · 2024Article
- Article
- Evaluation of Direct Detection Protocols for Poliovirus from Stool Samples of Acute Flaccid Paralysis Patients.Viruses · 2023Article
- Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study.Nature microbiology · 2023Article
- Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction.Microbiology spectrum · 2021Article
- Enterovirus Surveillance (EVSurv) in Germany.Microorganisms · 2021Article
- Article
- Detection and Typing of Human Enteroviruses from Clinical Samples by Entire-Capsid Next Generation Sequencing.Viruses · 2021Article
- Article
- Rapid and Sensitive Direct Detection and Identification of Poliovirus from Stool and Environmental Surveillance Samples by Use of Nanopore Sequencing.Journal of clinical microbiology · 2020Article
- Reference Echovirus 7 and 19 Genomes from Nigeria.Microbiology resource announcements · 2018Article
- Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection.BMC research notes · 2018Article
- Detection by Direct Next Generation Sequencing Analysis of Emerging Enterovirus D68 and C109 Strains in an Environmental Sample From Scotland.Frontiers in microbiology · 2018Article
- Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis.Journal of pathogens · 2017Article
- Defining the Enterovirus Diversity Landscape of a Fecal Sample: A Methodological Challenge?Viruses · 2016Review
- Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay.Advances in virology · 2016Article
- Enterovirus Species B Bias of RD Cell Line and Its Influence on Enterovirus Diversity Landscape.Food and environmental virology · 2015Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratory diagnosis has played a critical role in the Global Polio Eradication Initiative since 1988, by isolating and identifying poliovirus (PV) from stool specimens by using cell culture as a highly sensitive system to detect PV. In the present study, we aimed to develop a molecular method to detect PV directly from stool extracts, with a high efficiency comparable to that of cell culture. We developed a method to efficiently amplify the entire capsid coding region of human enteroviruses (EVs) including PV. cDNAs of the entire capsid coding region (3.9 kb) were obtained from as few as 50 copies of PV genomes. PV was detected from the cDNAs with an improved PV-specific real-time reverse transcription-PCR system and nucleotide sequence analysis of the VP1 coding region. For assay validation, we analyzed 84 stool extracts that were positive for PV in cell culture and detected PV genomes from 100% of the extracts (84/84 samples) with this method in combination with a PV-specific extraction method. PV could be detected in 2/4 stool extract samples that were negative for PV in cell culture. In PV-positive samples, EV species C viruses were also detected with high frequency (27% [23/86 samples]). This method would be useful for direct detection of PV from stool extracts without using cell culture.
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