ArticleJournal of clinical microbiology2015
Semiquantitative multiplexed tandem PCR for detection and differentiation of four Theileria orientalis genotypes in cattle.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Insights into the genetic diversity and population structure of prevalent Theileria orientalis in Bangladesh.PloS one · 2026Article
- Insight into oriental theileriosis: Indian perspective.Tropical animal health and production · 2025Review
- Molecular Identification and Genotyping of Theileria Orientalis Type 3 (Buffeli) Isolated from Cattle Using Nested-PCR Assay in Guilan Province, Iran.Archives of Razi Institute · 2021Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Targeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions.Microorganisms · 2020Article
- A Targeted "Next-Generation" Sequencing-Informatic Approach to Define Genetic Diversity inPathogens (Basel, Switzerland) · 2020Article
- Article
- An appraisal of oriental theileriosis and the Theileria orientalis complex, with an emphasis on diagnosis and genetic characterisation.Parasitology research · 2020Review
- Multiplexed Tandem PCR (MT-PCR) Assay Using the Major Piroplasm Surface Protein Gene for the Diagnosis of Theileria orientalis Infection in Cattle.Journal of clinical microbiology · 2018Article
- Inadequate Differentiation of Theileria orientalis Genotypes buffeli and ikeda in a Multiplexed Tandem PCR (MT-PCR) Assay Using the p23 Gene as a Marker.Journal of clinical microbiology · 2017Article
- Tick-borne diseases of bovines in Pakistan: major scope for future research and improved control.Parasites & vectors · 2015Review
- A review of Theileria diagnostics and epidemiology.International journal for parasitology. Parasites and wildlife · 2015Review
- Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes.Parasites & vectors · 2015Article
- Development and validation of a quantitative PCR assay using multiplexed hydrolysis probes for detection and quantification of Theileria orientalis isolates and differentiation of clinically relevant subtypes.Journal of clinical microbiology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oriental theileriosis is an emerging, tick-borne disease of bovines in the Asia-Pacific region and is caused by one or more genotypes of the Theileria orientalis complex. This study aimed to establish and validate a multiplexed tandem PCR (MT-PCR) assay using three distinct markers (major piroplasm surface protein, 23-kDa piroplasm membrane protein, and the first internal transcribed spacer of nuclear DNA), for the simultaneous detection and semiquantification of four genotypes (Buffeli, Chitose, Ikeda, and type 5) of the T. orientalis complex. Analytical specificity, analytical sensitivity, and repeatability of the established MT-PCR assay were assessed in a series of experiments. Subsequently, the assay was evaluated using 200 genomic DNA samples collected from cattle from farms on which oriental theileriosis outbreaks had occurred, and 110 samples from a region where no outbreaks had been reported. The results showed the MT-PCR assay specifically and reproducibly detected the expected genotypes (i.e., genotypes Buffeli, Chitose, Ikeda, and type 5) of the T. orientalis complex, reliably differentiated them, and was able to detect as little as 1 fg of genomic DNA from each genotype. The diagnostic specificity and sensitivity of the MT-PCR were estimated at 94.0% and 98.8%, respectively. The MT-PCR assay established here is a practical and effective diagnostic tool for the four main genotypes of T. orientalis complex in Australia and should assist studies of the epidemiology and pathophysiology of oriental theileriosis in the Asia-Pacific region.
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