ArticleCellular & molecular biology letters2014
Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture.
Article in Cellular & molecular biology letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 16 citations in OpenAlex.
- Protocol optimization to process mouse colon samples for single-nuclei RNA sequencing using FLEX library preparation.STAR protocols · 2026Article
- Transcriptome and Proteome Profiling of Primary Human Gastric Interstitial Cells of Cajal Predicts Pacemaker Networks.Journal of neurogastroenterology and motility · 2023Article
- Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Interstitial cells of Cajal in WJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2020Article
- The Potential for Gut Organoid Derived Interstitial Cells of Cajal in Replacement Therapy.International journal of molecular sciences · 2017Review
- Yangyin Runchang Decoction Improves Intestinal Motility in Mice with Atropine/Diphenoxylate-Induced Slow-Transit Constipation.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Use of anoctamin 1 (ANO1) to evaluate interstitial cells of Cajal in Hirschsprung's disease.Pediatric surgery international · 2016Article
- Recruiting endogenous stem cells: a novel therapeutic approach for erectile dysfunction.Asian journal of andrologyReview
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interstitial cells of Cajal (ICC) drive the slow wave-associated contractions in the small intestine. A commonly used marker for these cells is c-Kit, but another marker named Ano1 was recently described. This study uses single-cell RT-PCR, qPCR and immunohistochemistry to determine if Ano1 could be reliably used as a molecular marker for ICC in single-cell mRNA analysis. Here, we report on the relationship between the expression of c-Kit and Ano1 in single ICC in culture. We observed that Ano1 is expressed in more than 60% of the collected cells, whereas c-Kit is found only in 22% of the cells (n = 18). When we stained ICC primary cultures for c-KIT and ANO1 protein, we found complete co-localization in all the preparations. We propose that this difference is due to the regulation of c-Kit mRNA in culture. This regulation gives rise to low levels of its transcript, while Ano1 is expressed more prominently in culture on day 4. We also propose that Ano1 is more suitable for single-cell expression analysis as a marker for cell identity than c-Kit at the mRNA level. We hope this evidence will help to validate and increase the success of future studies characterizing single ICC expression patterns.
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