ArticlePloS one2014
The pro-apoptotic and pro-inflammatory effects of calprotectin on human periodontal ligament cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Calprotectin (S100A8/S100A9): a key protein between inflammation and cancer.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Pooled it
- The role of miR-3917/HLA-DRB1 in Th17 cell differentiation affects chronic periodontitis.Odontology · 2026Article
- The proteome of osteoblasts in a 3D culture perfusion bioreactor model compared with static conditions.Scientific reports · 2025Article
- Role of innate host defense proteins in oral cancerogenesis.Periodontology 2000 · 2024Review
- Insights into the Relationship between Periodontitis and Systemic Sclerosis Based on the New Periodontitis Classification (2018): A Cross-Sectional Study.Diagnostics (Basel, Switzerland) · 2024Article
- Impact of Non-Surgical Periodontal Treatment on the Concentration and Level of MRP-8/14 (Calprotectin) as an Inflammatory Biomarker in Women with Periodontitis and Rheumatoid Arthritis: A Quasi-Experimental Study.Diseases (Basel, Switzerland) · 2024Article
- S100a9 deficiency accelerates MDS-associated tumor escape via PD-1/PD-L1 overexpression.Acta biochimica et biophysica Sinica · 2023Article
- DecreasedPeerJ · 2023Article
- S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening established periodontitis: a cross-sectional study.BMC oral health · 2021Article
- S100A9 induces nucleus pulposus cell degeneration through activation of the NF-κB signaling pathway.Journal of cellular and molecular medicine · 2021Article
- Calprotectin in Lung Diseases.International journal of molecular sciences · 2021Review
- S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells.BioMed research international · 2020Article
- Apoptosis Transcriptional Profile Induced byMediators of inflammation · 2019Article
- Role of Calprotectin as a Biomarker in Periodontal Disease.Mediators of inflammation · 2019Review
- Calprotectin in rheumatic diseases.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- [Expression and effect of S100A9 in the periodontium of diabetic rats].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2016Article
- Article
- Fecal calprotectin evaluation in patients with ulcerative colitis.Digestive diseases and sciences · 2015Article
- Analysis of protein expression in periodontal pocket tissue: a preliminary study.Proteome science · 2015Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calprotectin, a heterodimer of S100A8 and S100A9 subunits, is associated with inflammatory disorders such as rheumatoid arthritis and cystic fibrosis. Although calprotectin levels are increased significantly in the gingival crevicular fluid (GCF) of periodontitis patients, its effects on periodontal ligament cells (PDLCs) remain largely unknown. The aim of this study was to evaluate calprotectin levels in the GCF of generalized aggressive periodontitis (AgP) patients and to investigate the effects of recombinant human calprotectin (rhS100A8/A9) and its subunits (rhS100A8 and rhS100A9) in PDLCs. Both the concentration and amount of crevicular calprotectin were significantly higher in the AgP group compared with healthy controls. In addition, the GCF calprotectin levels were correlated positively with clinical periodontal parameters including bleeding index, probing depth, and clinical attachment loss. rhS100A8/A9 promoted cell apoptosis, whereas rhS100A8 and rhS100A9 individually exerted little effect on apoptosis in PDLCs. rhS100A9 and rhS100A8/A9 increased the activation of nuclear factor-κB (NF-κB) by promoting the nuclear translocation of p65 in PDLCs, subsequently inducing expression of the pro-inflammatory cytokines IL-6, IL-8, TNFα, and COX2. Treatment with an NF-κB inhibitor partially reversed the rhS100A9- and rhS100A8/A9-induced upregulation of the pro-inflammatory cytokines. rhS100A9, and not rhS100A8, was mainly responsible for the pro-inflammatory role of calprotectin. Collectively, our results suggest that calprotectin promotes apoptosis and the inflammatory response in PDLCs via rhS100A9. These findings might help identify novel treatments for periodontitis.
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