ArticleACS nano2015
Bilayer-spanning DNA nanopores with voltage-switching between open and closed state.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- The Ion Permeability of DNA Nanotube Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Engineering DNA nanopores: from structural evolution to sensing and transport.Materials today. Bio · 2025Review
- Oriented triplex DNA as a synthetic receptor for transmembrane signal transduction.Nature communications · 2024Article
- Recent Developments in Single-Entity Electrochemistry.Analytical chemistry · 2024Review
- Exploring Novel Sensor Design Ideas through Concentration-Induced Conformational Changes in PEG Single Chains.Sensors (Basel, Switzerland) · 2024Article
- Nucleic acid amphiphiles: Synthesis, properties, and applications.Molecular therapy. Nucleic acids · 2023Review
- Structure and dynamics of an archetypal DNA nanoarchitecture revealed via cryo-EM and molecular dynamics simulations.Nature communications · 2023Article
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- All-Atom Molecular Dynamics Simulations of Membrane-Spanning DNA Origami Nanopores.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Surface Assembly of DNA Origami on a Lipid Bilayer Observed Using High-Speed Atomic Force Microscopy.Molecules (Basel, Switzerland) · 2022Review
- Triggered Assembly of a DNA-Based Membrane Channel.Journal of the American Chemical Society · 2022Article
- Therapeutic Applications of Programmable DNA Nanostructures.Micromachines · 2022Review
- A Surfactant Enables Efficient Membrane Spanning by Non-Aggregating DNA-Based Ion Channels.Molecules (Basel, Switzerland) · 2022Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Controlled dimerization of artificial membrane receptors for transmembrane signal transduction.Chemical science · 2021Article
- Construction of Metal-Organic Frameworks (MOFs)-Based Membranes and Their Ion Transport Applications.Small science · 2021Article
- Design, assembly, and characterization of membrane-spanning DNA nanopores.Nature protocols · 2021Article
- Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.APL bioengineering · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Membrane-spanning nanopores from folded DNA are a recent example of biomimetic man-made nanostructures that can open up applications in biosensing, drug delivery, and nanofluidics. In this report, we generate a DNA nanopore based on the archetypal six-helix-bundle architecture and systematically characterize it via single-channel current recordings to address several fundamental scientific questions in this emerging field. We establish that the DNA pores exhibit two voltage-dependent conductance states. Low transmembrane voltages favor a stable high-conductance level, which corresponds to an unobstructed DNA pore. The expected inner width of the open channel is confirmed by measuring the conductance change as a function of poly(ethylene glycol) (PEG) size, whereby smaller PEGs are assumed to enter the pore. PEG sizing also clarifies that the main ion-conducting path runs through the membrane-spanning channel lumen as opposed to any proposed gap between the outer pore wall and the lipid bilayer. At higher voltages, the channel shows a main low-conductance state probably caused by electric-field-induced changes of the DNA pore in its conformation or orientation. This voltage-dependent switching between the open and closed states is observed with planar lipid bilayers as well as bilayers mounted on glass nanopipettes. These findings settle a discrepancy between two previously published conductances. By systematically exploring a large space of parameters and answering key questions, our report supports the development of DNA nanopores for nanobiotechnology.
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Registered trials
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