ArticlePloS one2014
Reassessment of the role of TSC, mTORC1 and microRNAs in amino acids-meditated translational control of TOP mRNAs.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 protein.RNA (New York, N.Y.) · 2021Article
- Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 protein.bioRxiv : the preprint server for biology · 2021Article
- mTORC1 promotes TOP mRNA translation through site-specific phosphorylation of LARP1.Nucleic acids research · 2021Article
- Control of ribosomal protein synthesis by the Microprocessor complex.Science signaling · 2021Article
- Controversies around the function of LARP1.RNA biology · 2021Review
- Global analysis of LARP1 translation targets reveals tunable and dynamic features of 5' TOP motifs.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- LARP1 on TOP of ribosome production.Wiley interdisciplinary reviews. RNA · 2018Review
- Signaling Pathways Involved in the Regulation of mRNA Translation.Molecular and cellular biology · 2018Review
- Trans-acting translational regulatory RNA binding proteins.Wiley interdisciplinary reviews. RNA · 2018Review
- Genetics of tuberous sclerosis complex: implications for clinical practice.The application of clinical genetics · 2017Review
- The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise.Scientific reports · 2016Article
- New frontiers in translational control of the cancer genome.Nature reviews. Cancer · 2016Review
- Lysosomal recruitment of TSC2 is a universal response to cellular stress.Nature communications · 2016Article
- Article
- mTOR in health and in sickness.Journal of molecular medicine (Berlin, Germany) · 2015Review
- TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1.Scientific reports · 2015Article
- Milk--A Nutrient System of Mammalian Evolution Promoting mTORC1-Dependent Translation.International journal of molecular sciences · 2015Review
- A liaison between mTOR signaling, ribosome biogenesis and cancer.Biochimica et biophysica acta · 2015Review
- La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1).The Journal of biological chemistry · 2015Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TOP mRNAs encode components of the translational apparatus, and repression of their translation comprises one mechanism, by which cells encountering amino acid deprivation downregulate the biosynthesis of the protein synthesis machinery. This mode of regulation involves TSC as knockout of TSC1 or TSC2 rescued TOP mRNAs translation in amino acid-starved cells. The involvement of mTOR in translational control of TOP mRNAs is demonstrated by the ability of constitutively active mTOR to relieve the translational repression of TOP mRNA upon amino acid deprivation. Consistently, knockdown of this kinase as well as its inhibition by pharmacological means blocked amino acid-induced translational activation of these mRNAs. The signaling of amino acids to TOP mRNAs involves RagB, as overexpression of active RagB derepressed the translation of these mRNAs in amino acid-starved cells. Nonetheless, knockdown of raptor or rictor failed to suppress translational activation of TOP mRNAs by amino acids, suggesting that mTORC1 or mTORC2 plays a minor, if any, role in this mode of regulation. Finally, miR10a has previously been suggested to positively regulate the translation of TOP mRNAs. However, we show here that titration of this microRNA failed to downregulate the basal translation efficiency of TOP mRNAs. Moreover, Drosha knockdown or Dicer knockout, which carries out the first and second processing steps in microRNAs biosynthesis, respectively, failed to block the translational activation of TOP mRNAs by amino acid or serum stimulation. Evidently, these results are questioning the positive role of microRNAs in this mode of regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.