Evidence map›Paper›PMID 25337817›Full record

ArticlePloS one2014

An increase in CD3+CD4+CD25+ regulatory T cells after administration of umbilical cord-derived mesenchymal stem cells during sepsis.

Yu-Hua Chao, Han-Ping Wu, Kang-Hsi Wu, Yi-Giien Tsai, Ching-Tien Peng, Kuan-Chia Lin, Wan-Ru Chao, Maw-Sheng Lee, Yun-Ching Fu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 4 pooled it
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 4 syntheses or guidelines pooled it, 102 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 7 institutions in 1 country.

Yu-Hua ChaoInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Han-Ping WuDepartment of Pediatrics, Taichung Tzuchi Hospital, the Buddhist Medical Foundation, Taichung, Taiwan; Department of Medicine, Tzu Chi University, Hualien, Taiwan.
Kang-Hsi WuSchool of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Hemato-oncology, Children's Hospital, China Medical University Hospital, China Medical University, Taichung, Taiwan.
Yi-Giien TsaiSchool of Medicine, Chung Shan Medical University, Taichung, Taiwan; Departments of Pediatrics, Changhua Christian Hospital, Changhua, Taiwan; School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Ching-Tien PengSchool of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Hemato-oncology, Children's Hospital, China Medical University Hospital, China Medical University, Taichung, Taiwan; Department of Biotechnology and Bioinformatics, Asia University, Taichung, Taiwan.
Kuan-Chia LinSchool of Nursing, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan; Life-Course Epidemiology and Human Development Research Group, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan.
Wan-Ru ChaoInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Maw-Sheng LeeInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Yun-Ching FuInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Pediatrics, Taichung Veterans General Hospital, Taichung, Taiwan.
Chung Shan Medical University Hospital · TWAsia University · TWChina Medical University · TWKaohsiung Medical University · TWNational Taipei University of Nursing and Health Science · TWNational Yang Ming Chiao Tung University · TWTzu Chi University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sepsis remains an important cause of death worldwide, and vigorous immune responses during sepsis could be beneficial for bacterial clearance but at the price of collateral damage to self tissues. Mesenchymal stem cells (MSCs) have been found to modulate the immune system and attenuate sepsis. In the present study, MSCs derived from bone marrow and umbilical cord were used and compared. With a cecal ligation and puncture (CLP) model, the mechanisms of MSC-mediated immunoregulation during sepsis were studied by determining the changes of circulating inflammation-associated cytokine profiles and peripheral blood mononuclear cells 18 hours after CLP-induced sepsis. In vitro, bone marrow-derived MSCs (BMMSCs) and umbilical cord-derived MSCs (UCMSCs) showed a similar morphology and surface marker expression. UCMSCs had stronger potential for osteogenesis but lower for adipogenesis than BMMSCs. Compared with rats receiving PBS only after CLP, the percentage of circulating CD3+CD4+CD25+ regulatory T (Treg) cells and the ratio of Treg cells/T cells were elevated significantly in rats receiving MSCs. Further experiment regarding Treg cell function demonstrated that the immunosuppressive capacity of Treg cells from rats with CLP-induced sepsis was decreased, but could be restored by administration of MSCs. Compared with rats receiving PBS only after CLP, serum levels of interleukin-6 and tumor necrosis factor-α were significantly lower in rats receiving MSCs after CLP. There were no differences between BMMSCs and UCMSCs. In summary, this work provides the first in vivo evidence that administering BMMSCs or UCMSCs to rats with CLP-induced sepsis could increase circulating CD3+CD4+CD25+ Treg cells and Treg cells/T cells ratio, enhance Treg cell suppressive function, and decrease serum levels of interleukin-6 and tumor necrosis factor-α, suggesting the immunomodulatory association of Treg cells and MSCs during sepsis.

Indexed as

Mesenchymal Stem Cell TransplantationAdipocytesAnimalsAntigens, CDBone Marrow CellsCell DifferentiationCoculture TechniquesDisease Models, AnimalFetal BloodGene ExpressionHumansImmunomodulationInterleukin-6Lymphocyte CountMaleMesenchymal Stem CellsAntigens, CDInterleukin-6Tumor Necrosis Factor-alpha

Identifiers

PMID25337817
PMCPMC4206342
OpenAlexW1986721829

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.