ArticleDevelopment (Cambridge, England)2014
Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- An NKX2-3/5/6 homolog is required downstream of BMP signaling to pattern the sensory-adhesive organ of a tunicate larva.PLoS genetics · 2026Article
- A thyroid hormone-mediated opsin switch initiates metamorphosis in a proto-vertebrate.Science advances · 2026Article
- Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta.PLoS biology · 2026Article
- Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicatebioRxiv : the preprint server for biology · 2026Article
- A protocadherin mediates oral placode morphogenesis in the tunicate Ciona.Biology open · 2026Article
- An NKX2-5 homolog is required downstream of BMP signaling to pattern the sensory-adhesive organ of a tunicate larva.bioRxiv : the preprint server for biology · 2025Article
- Regulation of motor neuron differentiation in thebioRxiv : the preprint server for biology · 2025Article
- The tunicate Ciona.Nature methods · 2025Article
- Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors.The EMBO journal · 2025Article
- Development of cell labeling and gene editing tools in urochordateMarine life science & technology · 2025Article
- Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates.PLoS biology · 2025Article
- A gene regulatory network for specification and morphogenesis of a Mauthner Cell homolog in non-vertebrate chordates.Developmental biology · 2025Article
- A simple inland culture system provides insights into ascidian post-embryonic developmental physiology.Open biology · 2025Article
- CRISPR/Cas9 Protocols for Disrupting Gene Function in the Non-vertebrate Chordate Ciona.Integrative and comparative biology · 2024Review
- A change in cis-regulatory logic underlying obligate versus facultative muscle multinucleation in chordates.Development (Cambridge, England) · 2024Article
- The ion channel Anoctamin 10/TMEM16K coordinates organ morphogenesis across scales in the urochordate notochord.PLoS biology · 2024Article
- Specification and survival of post-metamorphic branchiomeric neurons in a non-vertebrate chordate.Development (Cambridge, England) · 2024Article
- A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordates.bioRxiv : the preprint server for biology · 2024Article
- Cis-regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona.Nature communications · 2024Article
- Snail Transcriptionally RepressesInternational journal of molecular sciences · 2024Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirt Ciona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of the Ebf (Collier/Olf/EBF) gene in hundreds of synchronized Ciona embryos. Phenotyping of transfected embryos in the 'F0' generation revealed that endogenous Ebf function is required for specification of Islet-expressing motor ganglion neurons and atrial siphon muscles. We demonstrate that CRISPR/Cas9 is sufficiently effective and specific to generate large numbers of embryos carrying mutations in a targeted gene of interest, which should allow for rapid screening of gene function in Ciona.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.