Evidence map›Paper›PMID 25336580›Full record

ArticleThe British journal of ophthalmology2015

Ciliary neurotrophic factor (CNTF)-mediated ganglion cell survival in a rodent model of non-arteritic anterior ischaemic optic neuropathy (NAION).

Michaela K Mathews, Yan Guo, Patricia Langenberg, Steven L Bernstein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The British journal of ophthalmology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Michaela K MathewsDepartment of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Yan GuoDepartment of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Patricia LangenbergDepartment of Epidemiology and Public Health, University of Maryland, Baltimore, Maryland, USA.
Steven L BernsteinDepartment of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
University of Maryland, Baltimore · US

Funding

Mechanisms of Optic Nerve Stroke NeuroprotectionR01EY015304 · NEI · UNIVERSITY OF MARYLAND BALTIMORE · PI BERNSTEIN, STEVEN L · 2004 to 2018
$4.7M
LIPOPROTEIN (A) IN VASCULAR DISEASES OF THE EYEK08EY016357 · NEI · UNIVERSITY OF MARYLAND BALTIMORE · PI MATHEWS, MICHAELA K · 2009 to 2013
$883k
NEI NIH HHS 5K08EY016357-05NEI NIH HHS K08 EY016357NEI NIH HHS R01 EY015304
6 · The paper itself

Abstract

backgroundCiliary neurotrophic factor (CNTF) has been shown to protect retinal ganglion cells (RGCs) in traumatic optic nerve injury. We sought to evaluate this neuroprotective effect of CNTF after an ischaemic event using rodent anterior ischaemic optic neuropathy (rAION), a mouse model of non-arteritic anterior ischaemic optic neuropathy (NAION).

methodsWe induced rAION in Thy1-cyan fluorescent protein (CFP) transgenic mice by exposing the optic nerve to frequency doubled neodymium yttrium aluminium garnet laser pulses following intravenous rose bengal injection. One day after rAION induction, an intravitreal injection of 0.75 μg CNTF or vehicle (sham injection) was given. Animals were euthanised on day 15 after induction, tissues isolated and CFP cells in the RGC layer were counted using stereology in flat-mounted retina. The average number of CFP-positive (CFP+) cells was determined for each study group and the percentages of RGC loss were compared between the different groups.

resultsTwo weeks after rAION induction, significantly more (CFP+) cells were preserved in CNTF-treated eyes than in sham-injected controls. Sham-treated animals showed a 58% loss of CFP+ cells. In contrast, CFP+ cell density in CNTF-treated eyes decreased by only 10%, when compared with untreated control eyes. This increased survival was statistically significant (p<0.05).

conclusionsCNTF exerts a neuroprotective effect in ischaemic optic nerve injury and promotes RGC survival, suggesting that CNTF may be effective in the clinical treatment of human NAION.

Indexed as

Disease Models, AnimalAnimalsArteritisCell CountCell SurvivalCiliary Neurotrophic FactorGreen Fluorescent ProteinsIntravitreal InjectionsMaleMiceMice, TransgenicNeuroprotective AgentsOptic Neuropathy, IschemicRetinal Ganglion CellsCiliary Neurotrophic FactorCyan Fluorescent ProteinGreen Fluorescent ProteinsNeuroprotective AgentsExperimental &#8211 animal modelsOptic NervePathologyTreatment Medical

Identifiers

PMID25336580
PMCPMC4477946
OpenAlexW2040154279

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.