ArticleThe Biochemical journal2014
Late stages of the synchronized macrophage fusion in osteoclast formation depend on dynamin.
Article in The Biochemical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Cell fusion in osteoclastogenesis.Biochemical Society transactions · 2025Review
- Moesin controls cell-cell fusion and osteoclast function.The Journal of cell biology · 2025Article
- Cell-cell fusion: To lose one life and begin another.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Article
- FORMATION OF MULTINUCLEATED OSTEOCLASTS DEPENDS ON AN OXIDIZED SPECIES OF CELL SURFACE ASSOCIATED LA PROTEIN.bioRxiv : the preprint server for biology · 2024Article
- Osteoclasts at Bone Remodeling: Order from Order.Results and problems in cell differentiation · 2024Review
- Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins.Stem cell research & therapy · 2023Article
- Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins.Research square · 2023Article
- Cell surface-bound La protein regulates the cell fusion stage of osteoclastogenesis.Nature communications · 2023Article
- LY450139 Inhibited Ti-Particle-Induced Bone Dissolution via Suppressing Notch and NF-κB Signaling Pathways.Calcified tissue international · 2022Article
- Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement.Cell proliferation · 2022Article
- YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion.Nature communications · 2021Article
- Heterogeneity and Actin Cytoskeleton in Osteoclast and Macrophage Multinucleation.International journal of molecular sciences · 2020Review
- Osteoclast Multinucleation: Review of Current Literature.International journal of molecular sciences · 2020Review
- Conserved pathway activation following xenogeneic, heterotypic fusion.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- How cells fuse.The Journal of cell biology · 2019Review
- Disruption of RAB-5 Increases EFF-1 Fusogen Availability at the Cell Surface and Promotes the Regenerative Axonal Fusion Capacity of the Neuron.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion.Developmental cell · 2018Article
- Nanopatterned bulk metallic glass-based biomaterials modulate macrophage polarization.Acta biomaterialia · 2018Article
- The effect of surface immobilized NBD peptide on osteoclastogenesis of rough titanium platesRSC advances · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Macrophage fusion that leads to osteoclast formation is one of the most important examples of cell-cell fusion in development, tissue homoeostasis and immune response. Protein machinery that fuses macrophages remains to be identified. In the present study, we explored the fusion stage of osteoclast formation for RAW macrophage-like murine cells and for macrophages derived from human monocytes. To uncouple fusion from the preceding differentiation processes, we accumulated fusion-committed cells in the presence of LPC (lysophosphatidylcholine) that reversibly blocks membrane merger. After 16 h, we removed LPC and observed cell fusion events that would normally develop within 16 h develop instead within 30-90 min. Thus, whereas osteoclastogenesis, generally, takes several days, our approach allowed us to focus on an hour in which we observe robust fusion between the cells. Complementing syncytium formation assay with a novel membrane merger assay let us study the synchronized fusion events downstream of a local merger between two plasma membranes, but before expansion of nascent membrane connections and complete unification of the cells. We found that the expansion of membrane connections detected as a growth of multinucleated osteoclasts depends on dynamin activity. In contrast, a merger between the plasma membranes of the two cells was not affected by inhibitors of dynamin GTPase. Thus dynamin that was recently found to control late stages of myoblast fusion also controls late stages of macrophage fusion, revealing an intriguing conserved mechanistic motif shared by diverse cell-cell fusion processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.