ArticleTissue engineering. Part A2015
Osseointegrative properties of electrospun hydroxyapatite-containing nanofibrous chitosan scaffolds.
Article in Tissue engineering. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration.TheScientificWorldJournal · 2017Pooled it
- Biomimetic Tissue Engineering Strategies for Craniofacial Applications.Biomimetics (Basel, Switzerland) · 2024Review
- Bone Regeneration Capabilities of Scaffolds Containing Chitosan and Nanometric Hydroxyapatite-Systematic Review Based on In Vivo Examinations.Biomimetics (Basel, Switzerland) · 2024Review
- The Diamond Concept Enigma: Recent Trends of Its Implementation in Cross-linked Chitosan-Based Scaffolds for Bone Tissue Engineering.ACS applied bio materials · 2023Review
- The advances in nanomedicine for bone and cartilage repair.Journal of nanobiotechnology · 2022Review
- The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers.Clinical oral investigations · 2021Article
- Predifferentiated Gingival Stem Cell-Induced Bone Regeneration in Rat Alveolar Bone Defect Model.Tissue engineering. Part A · 2021Article
- Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds.RSC advances · 2020Article
- OsteoBLAST: Computational Routine of Global Molecular Analysis Applied to Biomaterials Development.Frontiers in bioengineering and biotechnology · 2020Article
- Advancements and frontiers in nano-based 3D and 4D scaffolds for bone and cartilage tissue engineering.International journal of nanomedicine · 2019Review
- In Vivo Investigation into Effectiveness of Fe₃O₄/PLLA Nanofibers for Bone Tissue Engineering Applications.Polymers · 2018Article
- Plants and Their Bioactive Constituents in Mesenchymal Stem Cell-Based Periodontal Regeneration: A Novel Prospective.BioMed research international · 2018Review
- Silk Fibroin-Alginate-Hydroxyapatite Composite Particles in Bone Tissue Engineering Applications In Vivo.International journal of molecular sciences · 2017Article
- Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone.Marine drugs · 2015Review
- Improved cellular infiltration in electrospun fiber via engineered porosity.Tissue engineering · 2007Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Our long-term goal is to develop smart biomaterials that can facilitate regeneration of critical-size craniofacial lesions. In this study, we tested the hypothesis that biomimetic scaffolds electrospun from chitosan (CTS) will promote tissue repair and regeneration in a critical size calvarial defect. To test this hypothesis, we first compared in vitro ability of electrospun CTS scaffolds crosslinked with genipin (CTS-GP) to those of mineralized CTS-GP scaffolds containing hydroxyapatite (CTS-HA-GP), by assessing proliferation/metabolic activity and alkaline phosphatase (ALP) levels of murine mesenchymal stem cells (mMSCs). The cells' metabolic activity exhibited a biphasic behavior, indicative of initial proliferation followed by subsequent differentiation for all scaffolds. ALP activity of mMSCs, a surrogate measure of osteogenic differentiation, increased over time in culture. After 3 weeks in maintenance medium, ALP activity of mMSCs seeded onto CTS-HA-GP scaffolds was approximately two times higher than that of cells cultured on CTS-GP scaffolds. The mineralized CTS-HA-GP scaffolds were also osseointegrative in vivo, as inferred from the enhanced bone regeneration in a murine model of critical size calvarial defects. Tissue regeneration was evaluated over a 3 month period by microCT and histology (Hematoxylin and Eosin and Masson's Trichrome). Treatment of the lesions with CTS-HA-GP scaffolds induced a 38% increase in the area of de novo generated mineralized tissue area after 3 months, whereas CTS-GP scaffolds only led to a 10% increase. Preseeding with mMSCs significantly enhanced the regenerative capacity of CTS-GP scaffolds (by ∼3-fold), to 35% increase in mineralized tissue area after 3 months. CTS-HA-GP scaffolds preseeded with mMSCs yielded 45% new mineralized tissue formation in the defects. We conclude that the presence of HA in the CTS-GP scaffolds significantly enhances their osseointegrative capacity and that mineralized chitosan-based scaffolds crosslinked with genipin may represent a unique biomaterial with possible clinical relevance for the repair of critical calvarial bone defects.
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