Evidence map›Paper›PMID 25333938›Full record

ArticlePloS one2014

Changes in the bacterial microbiota in gut, blood, and lungs following acute LPS instillation into mice lungs.

Marc A Sze, Masashi Tsuruta, Shun-Wei Julia Yang, Yeni Oh, S F Paul Man, James C Hogg, Don D Sin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
112citing papers in PubMed, 2 pooled it
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

112 citing papers in PubMed, 2 syntheses or guidelines pooled it, 179 citations in OpenAlex.

  1. [Fecal Microbiota Transplantation: A Promising Avenue for Lung Cancer Therapy].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2025
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  6. The gut as a central hub for multi-organ crosstalk in aging.Cellular and molecular life sciences : CMLS · 2026
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52 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Marc A SzeHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada; Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Masashi TsurutaHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada.
Shun-Wei Julia YangHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada.
Yeni OhHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada.
S F Paul ManHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada; Departments of Medicine, Respiratory Division, University of British Columbia, Vancouver, BC, Canada.
James C HoggHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada; Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Don D SinHeart + Lung Innovation Centre at St. Paul's Hospital, University of British Columbia, Vancouver, BC, Canada; Departments of Medicine, Respiratory Division, University of British Columbia, Vancouver, BC, Canada.
St. Paul's Hospital · CAUniversity of British Columbia · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPrevious reports have shown that the gastrointestinal (GI) bacterial microbiota can have profound effects on the lungs, which has been described as the "gut-lung axis". However, whether a "lung-gut" axis exists wherein acute lung inflammation perturbs the gut and blood microbiota is unknown.

methodsAdult C57/Bl6 mice were exposed to one dose of LPS or PBS instillation (n=3 for each group) directly into lungs. Bacterial microbiota of the bronchoalveolar lavage fluid, blood, and cecum were determined using 454 pyrotag sequencing and quantitative polymerase chain reaction (qPCR) at 4 through 168 hours post-instillation. We then investigated the effects of oral neomycin and streptomycin (n=8) on the microbiota at 4 and 24 hours post LPS instillation versus control treatment (n=5 at baseline and 4 hours, n=7 at 24 hours).

resultsAt 24 hours post LPS instillation, the total bacterial count was significantly increased in the cecum (P<0.05); whereas the total bacterial count in blood was increased at 4, 48, and 72 hours (P<0.05). Antibiotic treatment reduced the total bacteria in blood but not in the cecum. The increase in total bacteria in the blood correlated with Phyllobacteriaceae OTU 40 and was significantly reduced in the blood for both antibiotic groups (P<0.05).

conclusionLPS instillation in lungs leads to acute changes in the bacterial microbiota in the blood and cecum, which can be modulated with antibiotics.

Indexed as

Acute Lung InjuryAnimalsBronchoalveolar Lavage FluidCecumLipopolysaccharidesLungMiceMicrobiotaNeomycinPhyllobacteriaceaeSerumStreptomycinLipopolysaccharidesNeomycinStreptomycin

Identifiers

PMID25333938
PMCPMC4205020
OpenAlexW2081107255

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.