ArticlePloS one2014
Expression of Cry1Ab and Cry2Ab by a polycistronic transgene with a self-cleavage peptide in rice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Translocation of Insecticidal Bt Protein in Transgrafted Plants.Biotech (Basel (Switzerland)) · 2025Article
- Molecular Characterization and Assessment of Insect Resistance of Transgenic Maize ZDRF-8.Plants (Basel, Switzerland) · 2025Article
- Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectors.Plant biotechnology journal · 2024Article
- FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double-stranded FAR in rice exhibits strong pest resistance.Plant biotechnology journal · 2022Article
- 2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of β-carotene, zeaxanthin, and ketocarotenoids in rice endosperm.Metabolic engineering communications · 2021Article
- A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize.Plant physiology · 2019Article
- Transgenic soybean expressing a thermostable phytase as substitution for feed additive phytase.Scientific reports · 2019Article
- A strong early acting wound-inducible promoter, RbPCD1pro, activates cryIAc expression within minutes of wounding to impart efficient protection against insects.Plant biotechnology journal · 2019Article
- What are farmers really planting? Measuring the presence and effectiveness of Bt cotton in Pakistan.PloS one · 2017Article
- Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene.Journal of Zhejiang University. Science. B · 2015Article
- Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect resistance to Bacillus thuringiensis (Bt) crystal protein is a major threat to the long-term use of transgenic Bt crops. Gene stacking is a readily deployable strategy to delay the development of insect resistance while it may also broaden insecticidal spectrum. Here, we report the creation of transgenic rice expressing discrete Cry1Ab and Cry2Ab simultaneously from a single expression cassette using 2A self-cleaving peptides, which are autonomous elements from virus guiding the polycistronic viral gene expression in eukaryotes. The synthetic coding sequences of Cry1Ab and Cry2Ab, linked by the coding sequence of a 2A peptide from either foot and mouth disease virus or porcine teschovirus-1, regardless of order, were all expressed as discrete Cry1Ab and Cry2Ab at high levels in the transgenic rice. Insect bioassays demonstrated that the transgenic plants were highly resistant to lepidopteran pests. This study suggested that 2A peptide can be utilized to express multiple Bt genes at high levels in transgenic crops.
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