ArticleBiomicrofluidics2014
Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Synergistic effect of Hypoxic Conditioning and Cell-Tethering Colloidal Gels enhanced Productivity of MSC Paracrine Factors and Accelerated Vessel Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Quantum Dots Mediated Imaging and Phototherapy in Cancer Spheroid Models: State of the Art and Perspectives.Pharmaceutics · 2022Review
- Vascularized Co-Culture Clusteroids of Primary Endothelial and Hep-G2 Cells Based on Aqueous Two-Phase Pickering Emulsions.Bioengineering (Basel, Switzerland) · 2022Article
- Modular multimodal platform for classical and high throughput light sheet microscopy.Scientific reports · 2022Article
- Comparison of Hydrogen Peroxide Secretion From Living Cells Cultured in Different Formats Using Hydrogel-Based LSPR Substrates.Frontiers in bioengineering and biotechnology · 2022Article
- Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids.RSC advances · 2020Article
- Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel.Scientific reports · 2020Article
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- A Human Liver-on-a-Chip Platform for Modeling Nonalcoholic Fatty Liver Disease.Advanced biosystems · 2019Article
- Study of oxygen tension variation within live tumor spheroids using microfluidic devices and multi-photon laser scanning microscopy.RSC advances · 2018Article
- Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment.Journal of the Royal Society, Interface · 2017Review
- Cytotoxic responses of carnosic acid and doxorubicin on breast cancer cells in butterfly-shaped microchips in comparison to 2D and 3D culture.Cytotechnology · 2017Article
- Imaging tissue-mimic with light sheet microscopy: A comparative guideline.Scientific reports · 2017Article
- Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device.Scientific reports · 2016Article
- Preface to Special Topic: Selected Papers from the Advances in Microfluidics and Nanofluidics 2014 Conference in Honor of Professor Hsueh-Chia Chang's 60th Birthday.Biomicrofluidics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a microfluidic device to culture cellular spheroids of controlled sizes and suitable for live cell imaging by selective plane illumination microscopy (SPIM). We cocultured human umbilical vein endothelial cells (HUVECs) within the spheroids formed by hepatocellular carcinoma cells, and studied the distributions of the HUVECs over time. We observed that the migration of HUVECs depended on the size of spheroids. In the spheroids of ∼200 μm diameters, HUVECs migrated outwards to the edges within 48 h; while in the spheroids of ∼250 μm diameters, there was no outward migration of the HUVECs up to 72 h. In addition, we studied the effects of pro-angiogenic factors, namely, vascular endothelial growth factor (VEGF) and fibroblast growth factor (β-FGF), on the migration of HUVECs in the carcinoma cell spheroid. The outward migration of HUVECs in 200 μm spheroids was hindered by the treatment with VEGF and β-FGF. Moreover, some of the HUVECs formed hollow lumen within 72 h under VEGF and β-FGF treatment. The combination of SPIM and microfluidic devices gives high resolution in both spatial and temporal domains. The observation of HUVECs in spheroids provides us insight on tumor vascularization, an ideal disease model for drug screening and fundamental studies.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.