Evidence map›Paper›PMID 25332432›Full record

ArticleJournal of lipid research2014

A comprehensive insight into the lipid composition of Myxococcus xanthus by UPLC-ESI-MS.

Wolfram Lorenzen, Kenan A J Bozhüyük, Niña S Cortina, Helge B Bode

Abstract read
In one paragraph

Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
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  4. Review
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  7. Review
  8. Comparative Lipidomic Analysis of Extracellular Vesicles Derived fromInternational journal of molecular sciences · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wolfram LorenzenMerck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
Kenan A J BozhüyükMerck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
Niña S CortinaCluster of Excellence Macromolecular Complexes, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.
Helge B BodeMerck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences (BMLS), Johann Wolfgang Goethe-Universität Frankfurt, D-60438 Frankfurt am Main, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Analysis of whole cell lipid extracts of bacteria by means of ultra-performance (UP)LC-MS allows a comprehensive determination of the lipid molecular species present in the respective organism. The data allow conclusions on its metabolic potential as well as the creation of lipid profiles, which visualize the organism's response to changes in internal and external conditions. Herein, we describe: i) a fast reversed phase UPLC-ESI-MS method suitable for detection and determination of individual lipids from whole cell lipid extracts of all polarities ranging from monoacylglycerophosphoethanolamines to TGs; ii) the first overview of a wide range of lipid molecular species in vegetative Myxococcus xanthus DK1622 cells; iii) changes in their relative composition in selected mutants impaired in the biosynthesis of α-hydroxylated FAs, sphingolipids, and ether lipids; and iv) the first report of ceramide phosphoinositols in M. xanthus, a lipid species previously found only in eukaryotes.

Indexed as

Lipid MetabolismBacterial ProteinsChromatography, High Pressure LiquidChromatography, Reverse-PhaseDatabases, ChemicalGas Chromatography-Mass SpectrometryLipidsMetabolomicsMolecular StructureMutationMyxococcus xanthusSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryBacterial ProteinsLipidsceramide phosphoinositolselectrospray ionization-mass spectrometryether lipidslipidomicslipid profilesmyxobacteriareversed phaseultra-performance liquid chromatography

Identifiers

PMID25332432
PMCPMC4242454

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.