ArticleJournal of bacteriology2015
Noncanonical cell-to-cell DNA transfer in Thermus spp. is insensitive to argonaute-mediated interference.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Rapid and Ultrasensitive Detection ofJournal of microbiology and biotechnology · 2025Article
- The PulE ATPase is required for twitching motility and DNA donation during Thermus thermophilus transjugation.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Article
- The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.Nucleic acids research · 2024Article
- Cell-to-cell non-conjugative plasmid transfer between Bacillus subtilis and lactic acid bacteria.Microbial biotechnology · 2023Article
- Deletion of the primase-polymerases encoding gene, located in a mobile element inFrontiers in microbiology · 2022Article
- Expression and Functional Analysis of the Argonaute Protein ofBiomolecules · 2021Article
- Exploring Phytochemicals for Combating Antibiotic Resistance in Microbial Pathogens.Frontiers in pharmacology · 2021Review
- Integrative and Conjugative Element ICETh1 Functions as a Pangenomic DNA Capture Module inMicroorganisms · 2020Article
- A thermostable DNA primase-polymerase from a mobile genetic element involved in defence against environmental DNA.Environmental microbiology · 2020Article
- Experimental approaches to tracking mobile genetic elements in microbial communities.FEMS microbiology reviews · 2020Review
- Prokaryotic Argonaute proteins: novel genome-editing tools?Nature reviews. Microbiology · 2018Review
- Article
- Article
- Autonomous Generation and Loading of DNA Guides by Bacterial Argonaute.Molecular cell · 2017Article
- The transjugation machinery of Thermus thermophilus: Identification of TdtA, an ATPase involved in DNA donation.PLoS genetics · 2017Article
- Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA.Nucleic acids research · 2015Article
- Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
Horizontal gene transfer drives the rapid evolution of bacterial populations. Classical processes that promote the lateral flow of genetic information are conserved throughout the prokaryotic world. However, some species have nonconserved transfer mechanisms that are not well known. This is the case for the ancient extreme thermophile Thermus thermophilus. In this work, we show that T. thermophilus strains are capable of exchanging large DNA fragments by a novel mechanism that requires cell-to-cell contacts and employs components of the natural transformation machinery. This process facilitates the bidirectional transfer of virtually any DNA locus but favors by 10-fold loci found in the megaplasmid over those in the chromosome. In contrast to naked DNA acquisition by transformation, the system does not activate the recently described DNA-DNA interference mechanism mediated by the prokaryotic Argonaute protein, thus allowing the organism to distinguish between DNA transferred from a mate and exogenous DNA acquired from unknown hosts. This Argonaute-mediated discrimination may be tentatively viewed as a strategy for safe sharing of potentially "useful" traits by the components of a given population of Thermus spp. without increasing the genome sizes of its individuals.
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