ArticleMethods in molecular biology (Clifton, N.J.)2015
In vivo axial loading of the mouse tibia.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Methodological aspects ofJournal of musculoskeletal & neuronal interactions · 2023Pooled it
- Experimental models of osteoarthritis: Advances, limitations and rational selection for translational research.Journal of orthopaedic translation · 2026Review
- Tart Cherry Supplementation Has Greater Osteogenic Effect on Adult and Aging Bone of C57BL/6 Female Mice than Treadmill Running.The Journal of nutrition · 2026Article
- Bone Multicellular Unit on a Chip (BMU-Chip) Subjected to Cyclic Mechanical Loading.ACS biomaterials science & engineering · 2026Article
- 3D Osteocyte Networks under Pulsatile Unidirectional Fluid Flow Stimuli (PUFFS).ACS biomaterials science & engineering · 2025Article
- A high throughput cell stretch device for investigating mechanobiologyAPL bioengineering · 2024Article
- Article
- Deletion of FNDC5/Irisin modifies murine osteocyte function in a sex-specific manner.bioRxiv : the preprint server for biology · 2024Article
- A synoptic literature review of animal models for investigating the biomechanics of knee osteoarthritis.Frontiers in bioengineering and biotechnology · 2024Review
- L-BAIBA Synergizes with Sub-Optimal Mechanical Loading to Promote New Bone Formation.JBMR plus · 2023Article
- Protein Kinase G2 Is Essential for Skeletal Homeostasis and Adaptation to Mechanical Loading in Male but Not Female Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Osteocyte Estrogen Receptor β (Ot-ERβ) Regulates Bone Turnover and Skeletal Adaptive Response to Mechanical Loading Differently in Male and Female Growing and Adult Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Microfluidic Co-culture Platforms for Studying Osteocyte Regulation of Other Cell Types under Dynamic Mechanical Stimulation.Current osteoporosis reports · 2022Review
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- Article
- Mechanical loading recovers bone but not muscle lost during unloading.NPJ microgravity · 2020Article
- Protective Effects of Controlled Mechanical Loading of Bone in C57BL6/J Mice Subject to Disuse.JBMR plus · 2020Article
- Murine Axial Compression Tibial Loading Model to Study Bone Mechanobiology: Implementing the Model and Reporting Results.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- Detection of the Strains Induced in Murine Tibias by Ex Vivo Uniaxial Loading with Different Sensors.Sensors (Basel, Switzerland) · 2019Article
- Low-level cyclic tibial compression attenuates early osteoarthritis progression after joint injury in mice.Osteoarthritis and cartilage · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Noninvasive methods to apply controlled, cyclic loads to the living skeleton are used as anabolic procedures to stimulate new bone formation in adults and enhance bone mass accrual in growing animals. These methods are also invaluable for understanding bone signaling pathways. Our focus here is on a particular loading model: in vivo axial compression of the mouse tibia. An advantage of loading the tibia is that changes are present in both the cancellous envelope of the proximal tibia and the cortical bone of the tibial diaphysis. To load the tibia of the mouse axially in vivo, a cyclic compressive load is applied up to five times a week to a single tibia per mouse for a duration lasting from 1 day to 6 weeks. With the contralateral limb as an internal control, the anabolic response of the skeleton to mechanical stimuli can be studied in a pairwise experimental design. Here, we describe the key parameters that must be considered before beginning an in vivo mouse tibial loading experiment, including methods for in vivo strain gauging of the tibial midshaft, and then we describe general methods for loading the mouse tibia for an experiment lasting multiple days.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.