ArticlePLoS neglected tropical diseases2014
Systems biology studies of adult paragonimus lung flukes facilitate the identification of immunodominant parasite antigens.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Clinical evaluation of recombinant antigen ELISAs and broad-range helminth PCRs for the diagnosis of human paragonimiasis in Gabon.Tropical medicine and health · 2026Article
- Immunoblotting Identification of Diagnostic Antigens ofTropical medicine and infectious disease · 2023Article
- The proteome of extracellular vesicles of the lung fluke Paragonimus kellicotti produced in vitro and in the lung cyst.Scientific reports · 2023Article
- EndemicFood and waterborne parasitology · 2023Review
- General overview of the current status of human foodborne trematodiasis.Parasitology · 2022Review
- A clinical review of human disease due toParasitology · 2022Review
- Comparative proteomics of adult Paragonimus kellicotti excretion/secretion products released in vitro or present in the lung cyst nodule.PLoS neglected tropical diseases · 2022Article
- Characterization and localization of antigens for serodiagnosis of human paragonimiasis.Parasitology research · 2021Article
- Comparative genomics and transcriptomics of 4 Paragonimus species provide insights into lung fluke parasitism and pathogenesis.GigaScience · 2020Article
- Transcriptomic analysis of hookworm Ancylostoma ceylanicum life cycle stages reveals changes in G-protein coupled receptor diversity associated with the onset of parasitism.International journal for parasitology · 2020Article
- Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.Scientific reports · 2018Article
- Cysteine proteases as digestive enzymes in parasitic helminths.PLoS neglected tropical diseases · 2018Review
- Surveillance and diagnosis of zoonotic foodborne parasites.Food science & nutrition · 2018Review
- Direct experimental manipulation of intestinal cells in Ascaris suum, with minor influences on the global transcriptome.International journal for parasitology · 2017Article
- Genomes of Fasciola hepatica from the Americas Reveal Colonization with Neorickettsia Endobacteria Related to the Agents of Potomac Horse and Human Sennetsu Fevers.PLoS genetics · 2017Article
- Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini.Parasites & vectors · 2016Article
- The omic approach to parasitic trematode research-a review of techniques and developments within the past 5 years.Parasitology research · 2016Review
- An Integrated Multiomics Approach to Identify Candidate Antigens for Serodiagnosis of Human Onchocerciasis.Molecular & cellular proteomics : MCP · 2015Article
- Identification and evaluation of vaccine candidate antigens from the poultry red mite (Dermanyssus gallinae).International journal for parasitology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundParagonimiasis is a food-borne trematode infection acquired by eating raw or undercooked crustaceans. It is a major public health problem in the far East, but it also occurs in South Asia, Africa, and in the Americas. Paragonimus worms cause chronic lung disease with cough, fever and hemoptysis that can be confused with tuberculosis or other non-parasitic diseases. Treatment is straightforward, but diagnosis is often delayed due to a lack of reliable parasitological or serodiagnostic tests. Hence, the purpose of this study was to use a systems biology approach to identify key parasite proteins that may be useful for development of improved diagnostic tests. METHODOLOGY/PRINCIPAL
findingsThe transcriptome of adult Paragonimus kellicotti was sequenced with Illumina technology. Raw reads were pre-processed and assembled into 78,674 unique transcripts derived from 54,622 genetic loci, and 77,123 unique protein translations were predicted. A total of 2,555 predicted proteins (from 1,863 genetic loci) were verified by mass spectrometric analysis of total worm homogenate, including 63 proteins lacking homology to previously characterized sequences. Parasite proteins encoded by 321 transcripts (227 genetic loci) were reactive with antibodies from infected patients, as demonstrated by immunoaffinity purification and high-resolution liquid chromatography-mass spectrometry. Serodiagnostic candidates were prioritized based on several criteria, especially low conservation with proteins in other trematodes. Cysteine proteases, MFP6 proteins and myoglobins were abundant among the immunoreactive proteins, and these warrant further study as diagnostic candidates.
conclusionsThe transcriptome, proteome and immunolome of adult P. kellicotti represent a major advance in the study of Paragonimus species. These data provide a powerful foundation for translational research to develop improved diagnostic tests. Similar integrated approaches may be useful for identifying novel targets for drugs and vaccines in the future.
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