ArticlePloS one2014
Ionising radiation immediately impairs synaptic plasticity-associated cytoskeletal signalling pathways in HT22 cells and in mouse brain: an in vitro/in vivo comparison study.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Tumor-infiltrating nerves: unraveling the role of cancer neuroscience in tumorigenesis, disease progression, and emerging therapies.Discover oncology · 2025Review
- Drug protection against radiation-induced neurological injury: mechanisms and developments.Archives of toxicology · 2025Review
- Research trends on cancer neuroscience: a bibliometric and visualized analysis.Frontiers in neuroscience · 2024Article
- Review
- Advances in the study of the molecular biological mechanisms of radiation-induced brain injury.American journal of cancer research · 2023Review
- Ionizing radiation alters functional neurotransmission inFrontiers in cellular neuroscience · 2023Article
- Chronic Home Radon Exposure Is Associated with Higher Inflammatory Biomarker Concentrations in Children and Adolescents.International journal of environmental research and public health · 2022Article
- Hippocampus sparing volumetric modulated arc therapy in patients with loco-regionally advanced oropharyngeal cancer.Physics and imaging in radiation oncology · 2022Article
- 0.5‑Gy X‑ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts.Molecular medicine reports · 2021Article
- Low-dose X-ray irradiation induces morphological changes and cytoskeleton reorganization in osteoblasts.Experimental and therapeutic medicine · 2020Article
- Irradiation-Induced Upregulation of miR-711 Inhibits DNA Repair and Promotes Neurodegeneration Pathways.International journal of molecular sciences · 2020Article
- Down-Regulation of miR-23a-3p Mediates Irradiation-Induced Neuronal Apoptosis.International journal of molecular sciences · 2020Article
- Combined Treatment with Low-Dose Ionizing Radiation and Ketamine Induces Adverse Changes in CA1 Neuronal Structure in Male Murine Hippocampi.International journal of molecular sciences · 2019Article
- Shedding light into memories under circadian rhythm system control.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- White Matter is the Predilection Site of Late-Delayed Radiation-Induced Brain Injury in Non-Human Primates.Radiation research · 2019Article
- Epicatechin Prevents Methamphetamine-Induced Neuronal Cell Death via Inhibition of ER Stress.Biomolecules & therapeutics · 2019Article
- Ameliorating mitochondrial dysfunction restores carbon ion-induced cognitive deficits via co-activation of NRF2 and PINK1 signaling pathway.Redox biology · 2018Article
- TNFα affects CREB-mediated neuroprotective signaling pathways of synaptic plasticity in neurons as revealed by proteomics and phospho-proteomics.Oncotarget · 2017Article
- Advances in Roles of miR-132 in the Nervous System.Frontiers in pharmacology · 2017Review
- Chronic low-dose-rate ionising radiation affects the hippocampal phosphoproteome in the ApoE-/- Alzheimer's mouse model.Oncotarget · 2016Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Patients suffering from brain malignancies are treated with high-dose ionising radiation. However, this may lead to severe learning and memory impairment. Preventive treatments to minimise these side effects have not been possible due to the lack of knowledge of the involved signalling pathways and molecular targets. Mouse hippocampal neuronal HT22 cells were irradiated with acute gamma doses of 0.5 Gy, 1.0 Gy and 4.0 Gy. Changes in the cellular proteome were investigated by isotope-coded protein label technology and tandem mass spectrometry after 4 and 24 hours. To compare the findings with the in vivo response, male NMRI mice were irradiated on postnatal day 10 with a gamma dose of 1.0 Gy, followed by evaluation of the cellular proteome of hippocampus and cortex 24 hours post-irradiation. Analysis of the in vitro proteome showed that signalling pathways related to synaptic actin-remodelling were significantly affected at 1.0 Gy and 4.0 Gy but not at 0.5 Gy after 4 and 24 hours. We observed radiation-induced reduction of the miR-132 and Rac1 levels; miR-132 is known to regulate Rac1 activity by blocking the GTPase-activating protein p250GAP. In the irradiated hippocampus and cortex we observed alterations in the signalling pathways similar to those in vitro. The decreased expression of miR-132 and Rac1 was associated with an increase in hippocampal cofilin and phospho-cofilin. The Rac1-Cofilin pathway is involved in the modulation of synaptic actin filament formation that is necessary for correct spine and synapse morphology to enable processes of learning and memory. We suggest that acute radiation exposure leads to rapid dendritic spine and synapse morphology alterations via aberrant cytoskeletal signalling and processing and that this is associated with the immediate neurocognitive side effects observed in patients treated with ionising radiation.
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