ArticlePLoS genetics2014
The chromosomal association of the Smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Article
- Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks.Nature communications · 2026Article
- The SMC5/SMC6 complex is critical for resolving R-loop-induced transcription-replication conflicts.Nucleic acids research · 2026Article
- Structural and mechanistic perspectives on Nse5/6 regulation of the Smc5/6 complex.Frontiers in molecular biosciences · 2026Review
- DNA double-strand break movement in heterochromatin depends on the histone acetyltransferase dGcn5.Nucleic acids research · 2024Article
- Human Smc5/6 recognises transcription-generated positive DNA supercoils.Nature communications · 2024Article
- RAD18 directs DNA double-strand break repair by homologous recombination to post-replicative chromatin.Nucleic acids research · 2024Article
- Crucial role of the NSE1 RING domain in Smc5/6 stability and FANCM-independent fork progression.Cellular and molecular life sciences : CMLS · 2024Article
- The SMC5/6 complex: folding chromosomes back into shape when genomes take a break.Nucleic acids research · 2024Review
- Article
- Smc5/6 silences episomal transcription by a three-step function.Nature structural & molecular biology · 2022Article
- Cohesin-dependent chromosome loop extrusion is limited by transcription and stalled replication forks.Science advances · 2022Article
- Sister chromatid-sensitive Hi-C to map the conformation of replicated genomes.Nature protocols · 2022Review
- The histone H4 lysine 20 demethylase DPY-21 regulates the dynamics of condensin DC binding.Journal of cell science · 2022Article
- HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts.Experimental and therapeutic medicine · 2021Article
- Acute ethanol stress induces sumoylation of conserved chromatin structural proteins inMolecular biology of the cell · 2021Article
- Natural variants suppress mutations in hundreds of essential genes.Molecular systems biology · 2021Article
- Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo.eLife · 2021Article
- Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction.Molecular cell · 2020Article
- The Smc5/6 Core Complex Is a Structure-Specific DNA Binding and Compacting Machine.Molecular cell · 2020Article
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12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cohesin complex, which is essential for sister chromatid cohesion and chromosome segregation, also inhibits resolution of sister chromatid intertwinings (SCIs) by the topoisomerase Top2. The cohesin-related Smc5/6 complex (Smc5/6) instead accumulates on chromosomes after Top2 inactivation, known to lead to a buildup of unresolved SCIs. This suggests that cohesin can influence the chromosomal association of Smc5/6 via its role in SCI protection. Using high-resolution ChIP-sequencing, we show that the localization of budding yeast Smc5/6 to duplicated chromosomes indeed depends on sister chromatid cohesion in wild-type and top2-4 cells. Smc5/6 is found to be enriched at cohesin binding sites in the centromere-proximal regions in both cell types, but also along chromosome arms when replication has occurred under Top2-inhibiting conditions. Reactivation of Top2 after replication causes Smc5/6 to dissociate from chromosome arms, supporting the assumption that Smc5/6 associates with a Top2 substrate. It is also demonstrated that the amount of Smc5/6 on chromosomes positively correlates with the level of missegregation in top2-4, and that Smc5/6 promotes segregation of short chromosomes in the mutant. Altogether, this shows that the chromosomal localization of Smc5/6 predicts the presence of the chromatid segregation-inhibiting entities which accumulate in top2-4 mutated cells. These are most likely SCIs, and our results thus indicate that, at least when Top2 is inhibited, Smc5/6 facilitates their resolution.
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