ArticlePloS one2014
Basophil activation test using recombinant allergens: highly specific diagnostic method complementing routine tests in wasp venom allergy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Biomarkers for the Molecular Diagnosis of IgE-Mediated Hymenoptera Venom Allergy in Clinical Practice.International journal of molecular sciences · 2024Review
- Hymenoptera venom allergy in children.Italian journal of pediatrics · 2024Review
- Multiplex basophil activation tests for allergy diagnosis: present and future applications.Frontiers in allergy · 2024Review
- Basophil activation test in Hymenoptera venom allergy.Allergologie select · 2024Review
- Basophil Activation Test with Different Polyethylene Glycols in Patients with Suspected PEG Hypersensitivity Reactions.International journal of molecular sciences · 2022Article
- Diagnosis ofBiomedicines · 2022Review
- Wasp Venom Biochemical Components and Their Potential in Biological Applications and Nanotechnological Interventions.Toxins · 2021Review
- Antigen 5 Allergens of Hymenoptera Venoms and Their Role in Diagnosis and Therapy of Venom Allergy.Current allergy and asthma reports · 2020Review
- Diagnostics in Hymenoptera venom allergy: current concepts and developments with special focus on molecular allergy diagnostics.Allergo journal international · 2017Article
- Pros and Cons of Clinical Basophil Testing (BAT).Current allergy and asthma reports · 2016Review
- A novel approach to predict cetuximab-induced hypersensitivity reaction: detection of drug-specific IgE on basophils.Cancer medicine · 2016Article
- Review
- Anaphylaxis to insect venom allergens: role of molecular diagnostics.Current allergy and asthma reports · 2015Review
- Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology.The journal of venomous animals and toxins including tropical diseases · 2015Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSkin testing can expose allergic subjects to potential systemic reactions, sensitization against unrelated proteins, and increased risk of future sting reactions. Therefore the continuous improvement of in vitro diagnostic methods is desirable. Recombinant allergens have been shown to improve the sensitivity of specific IgE (sIgE) detection in vitro whilst no data is available regarding their application and reliability in basophil activation test (BAT). Here we aimed to compare the specificity and sensitivity of recombinant allergens Ves v 1, Ves v 2, Ves v 3 and Ves v 5 in both specific IgE (sIgE) detection in vitro and basophil activation test.
methodssIgE detection by ELISA or ImmunoCAP and BAT towards the panel of recombinant allergens Ves v 1, Ves v 2, Ves v 3 and Ves v 5 were performed in 43 wasp venom allergic patients with a history of anaphylactic reaction and sIgE seropositivity, as well as 17 controls defined as subjects with a history of repetitive wasp stings but absence of any allergic symptom.
resultsThe BAT performed with the recombinant allergens Ves v 1, Ves v 2, Ves v 3 and Ves v 5 markedly improved the specificity of diagnosis in wasp venom allergic subjects when compared to the respective sIgE detection in serum.
conclusionsBAT performed with the recombinant allergens Ves v 5, Ves v 3 and Ves v 1 provides an emerging highly specific in vitro method for the detection of wasp venom allergy, compared to the sIgE detection. Recombinant allergens applied to BAT represent a step forward in developing reliable in vitro tests for specific diagnosis of allergy.
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