ArticlePloS one2014
Primary human osteoblasts in response to 25-hydroxyvitamin D3, 1,25-dihydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Nutritional Deficiencies and Reduced Bone Mineralization in Ulcerative Colitis.Journal of clinical medicine · 2025Review
- Effects of the Interaction between Dietary Vitamin DAnimals : an open access journal from MDPI · 2024Article
- Review
- 25-hydroxycholecalciferol reverses heat induced alterations in bone quality in finisher broilers associated with effects on intestinal integrity and inflammation.Journal of animal science and biotechnology · 2021Article
- Effects of 1,25 and 24,25 Vitamin D on Corneal Fibroblast VDR and Vitamin D Metabolizing and Catabolizing Enzymes.Current eye research · 2021Article
- Longitudinal assessment of salivary vitamin D binding protein during orthodontic tooth movement.BMC oral health · 2021Article
- Dietary supplementation of 25-hydroxycholecalciferol increases tibial mass by suppression bone resorption in meat ducks.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2020Article
- Vitamin K2 Modulates Vitamin D-Induced Mechanical Properties of Human 3D Bone Spheroids In Vitro.JBMR plus · 2020Article
- Preclinical safety and efficacy of 24R,25-dihydroxyvitamin DJournal of orthopaedic translation · 2020Article
- Reconstructed human skin shows epidermal invagination towards integrated neopapillae indicating early hair follicle formation in vitro.Journal of tissue engineering and regenerative medicine · 2020Article
- Burst, Short, and Sustained Vitamin DInternational journal of molecular sciences · 2020Article
- Effect of dietary 25-hydroxycholecalciferol on the sternal mass of meat ducks under different vitamin regimens.Poultry science · 2020Article
- Vitamin D receptor and metabolite effects on corneal epithelial cell gap junction proteins.Experimental eye research · 2019Article
- Article
- Endothelial cells enhance adipose mesenchymal stromal cell-mediated matrix contraction via ALK receptors and reduced follistatin: Potential role of endothelial cells in skin fibrosis.Journal of cellular physiology · 2018Article
- Effects of 1,25 and 24,25 Vitamin D on Corneal Epithelial Proliferation, Migration and Vitamin D Metabolizing and Catabolizing Enzymes.Scientific reports · 2017Article
- Co-expression of 1α-hydroxylase and vitamin D receptor in human articular chondrocytes.BMC musculoskeletal disorders · 2017Article
- Acute Homeostatic Changes Following Vitamin DJournal of the Endocrine Society · 2017Article
- Chronic kidney disease and vitamin D metabolism in human bone marrow-derived MSCs.Annals of the New York Academy of Sciences · 2017Review
- 25-Hydroxyvitamin DScientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most biologically active metabolite 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) has well known direct effects on osteoblast growth and differentiation in vitro. The precursor 25-hydroxyvitamin D3 (25(OH)D3) can affect osteoblast function via conversion to 1,25(OH)2D3, however, it is largely unknown whether 25(OH)D3 can affect primary osteoblast function on its own. Furthermore, 25(OH)D3 is not only converted to 1,25(OH)2D3, but also to 24R,25-dihydroxyvitamin D3 (24R,25(OH)2D3) which may have bioactivity as well. Therefore we used a primary human osteoblast model to examine whether 25(OH)D3 itself can affect osteoblast function using CYP27B1 silencing and to investigate whether 24R,25(OH)2D3 can affect osteoblast function. We showed that primary human osteoblasts responded to both 25(OH)D3 and 1,25(OH)2D3 by reducing their proliferation and enhancing their differentiation by the increase of alkaline phosphatase, osteocalcin and osteopontin expression. Osteoblasts expressed CYP27B1 and CYP24 and synthesized 1,25(OH)2D3 and 24R,25(OH)2D3 dose-dependently. Silencing of CYP27B1 resulted in a decline of 1,25(OH)2D3 synthesis, but we observed no significant differences in mRNA levels of differentiation markers in CYP27B1-silenced cells compared to control cells after treatment with 25(OH)D3. We demonstrated that 24R,25(OH)2D3 increased mRNA levels of alkaline phosphatase, osteocalcin and osteopontin. In addition, 24R,25(OH)2D3 strongly increased CYP24 mRNA. In conclusion, the vitamin D metabolites 25(OH)D3, 1,25(OH)2D3 and 24R,25(OH)2D3 can affect osteoblast differentiation directly or indirectly. We showed that primary human osteoblasts not only respond to 1,25(OH)2D3, but also to 24R,25(OH)2D3 by enhancing osteoblast differentiation. This suggests that 25(OH)D3 can affect osteoblast differentiation via conversion to the active metabolite 1,25(OH)2D3, but also via conversion to 24R,25(OH)2D3. Whether 25(OH)D3 has direct actions on osteoblast function needs further investigation.
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