ArticlePLoS pathogens2014
Co-opted oxysterol-binding ORP and VAP proteins channel sterols to RNA virus replication sites via membrane contact sites.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 114 citations in OpenAlex.
- Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.The New phytologist · 2026Article
- Targeted knockout of a host peroxisomal peptidase confers field resistance to maize lethal necrosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A Highly Conserved SNARE-Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking.Plant biotechnology journal · 2026Article
- Beyond Viral Restriction: The Metabolic Dimensions of Interferon-Stimulated Genes in Antiviral Immunity.Viruses · 2026Review
- RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.Nature communications · 2025Article
- Proviral role of ATG2 autophagy related protein in tomato bushy stunt virus replication through bulk phospholipid transfer into the viral replication organelle.Molecular biology of the cell · 2024Article
- Positive-strand RNA virus replication organelles at a glance.Journal of cell science · 2024Review
- Logistics of defense: The contribution of endomembranes to plant innate immunity.The Journal of cell biology · 2024Review
- Viral regulation of organelle membrane contact sites.PLoS biology · 2024Article
- Subversion of selective autophagy for the biogenesis of tombusvirus replication organelles inhibits autophagy.PLoS pathogens · 2024Article
- Activation of MAPK-mediated immunity by phosphatidic acid in response to positive-strand RNA viruses.Plant communications · 2024Article
- Carnation Italian Ringspot Virus p36 Expression Induces Mitochondrial Fission and Respiratory Chain Complex Impairment in Yeast.International journal of molecular sciences · 2023Article
- Dynamin-related protein 2 interacts with the membrane-associated methyltransferase domain of plantago asiatica mosaic virus replicase and promotes viral replication.Virus research · 2023Article
- Article
- Manipulation of the Cellular Membrane-Cytoskeleton Network for RNA Virus Replication and Movement in Plants.Viruses · 2023Review
- A sword or a buffet: plant endomembrane system in viral infections.Frontiers in plant science · 2023Review
- Reticulons 3 and 6 interact with viral movement proteins.Molecular plant pathology · 2022Article
- Race against Time between the Virus and Host: Actin-Assisted Rapid Biogenesis of Replication Organelles is Used by TBSV to Limit the Recruitment of Cellular Restriction Factors.Journal of virology · 2022Article
- Effects of the Noncoding Subgenomic RNA of Red Clover Necrotic Mosaic Virus in Virus Infection.Journal of virology · 2022Article
- Phytosterol metabolism in plant positive-strand RNA virus replication.Plant cell reports · 2022Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses recruit cellular membranes and subvert cellular proteins involved in lipid biosynthesis to build viral replicase complexes and replication organelles. Among the lipids, sterols are important components of membranes, affecting the shape and curvature of membranes. In this paper, the tombusvirus replication protein is shown to co-opt cellular Oxysterol-binding protein related proteins (ORPs), whose deletion in yeast model host leads to decreased tombusvirus replication. In addition, tombusviruses also subvert Scs2p VAP protein to facilitate the formation of membrane contact sites (MCSs), where membranes are juxtaposed, likely channeling lipids to the replication sites. In all, these events result in redistribution and enrichment of sterols at the sites of viral replication in yeast and plant cells. Using in vitro viral replication assay with artificial vesicles, we show stimulation of tombusvirus replication by sterols. Thus, co-opting cellular ORP and VAP proteins to form MCSs serves the virus need to generate abundant sterol-rich membrane surfaces for tombusvirus replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.