ArticlePloS one2014
Prolonged mechanical stretch initiates intracellular calcium oscillations in human mesenchymal stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Bone targeted nano-drug and nano-delivery.Bone research · 2024Review
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- Short-term transcriptomic response to plasma membrane injury.Scientific reports · 2021Article
- Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation.Molecular biology of the cell · 2019Article
- Static Preload Inhibits Loading-Induced Bone Formation.JBMR plus · 2019Article
- Mechanical features of endothelium regulate cell adhesive molecule-induced calcium response in neutrophils.APL bioengineering · 2019Article
- TRPV4-mediated calcium signaling in mesenchymal stem cells regulates aligned collagen matrix formation and vinculin tension.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Focused ultrasound activates voltage-gated calcium channels through depolarizing TRPC1 sodium currents in kidney and skeletal muscle.Theranostics · 2019Article
- Mechanical stretch and chronotherapeutic techniques for progenitor cell transplantation and biomaterials.BioMedicine · 2018Article
- Role of membrane-tension gated CaJournal of cell science · 2018Article
- Low-Intensity Ultrasound Modulates CaAnnals of biomedical engineering · 2018Article
- Role of Stem Cells in Pathophysiology and Therapy of Spondyloarthropathies-New Therapeutic Possibilities?International journal of molecular sciences · 2017Review
- On the origin and impact of mesenchymal stem cell heterogeneity: new insights and emerging tools for single cell analysis.European cells & materials · 2017Review
- Article
- Mechanically Induced Chromatin Condensation Requires Cellular Contractility in Mesenchymal Stem Cells.Biophysical journal · 2016Article
- RNA-induced silencing attenuates G protein-mediated calcium signals.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Mesenchymal stem cells (MSCs) are a promising candidate for cell-based therapy in regenerative medicine. These stem cells can interact with their mechanical microenvironment to control their functions. External mechanical cues can be perceived and transmitted into intracellular calcium dynamics to regulate various cellular processes. Recent studies indicate that human MSCs (hMSCs) exhibit a heterogeneous nature with a subset of hMSCs lacking spontaneous calcium oscillations. In this study, we studied whether and how external mechanical tension can be applied to trigger and restore the intracellular calcium oscillation in these hMSCs lacking spontaneous activities. Utilizing the fluorescence resonance energy transfer (FRET) based calcium biosensor, we found that this subpopulation of hMSCs can respond to a prolonged mechanical stretch (PMS). Further results revealed that the triggering of calcium oscillations in these cells is dependent on the calcium influx across the plasma membrane, as well as on both cytoskeletal supports, myosin light chain kinase (MLCK)-driven actomyosin contractility, and phospholipase C (PLC) activity. Thus, our report confirmed that mechanical tension can govern the intracellular calcium oscillation in hMSCs, possibly via the control of the calcium permeability of channels at the plasma membrane. Our results also provide novel mechanistic insights into how hMSCs sense mechanical environment to regulate cellular functions.
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Registered trials
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