ArticleVaccines2014
HSP70 promoter-driven activation of gene expression for immunotherapy using gold nanorods and near infrared light.
Article in Vaccines, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Nanoparticle-Decorated Biomimetic Extracellular Matrix for Cell Nanoencapsulation and Regulation.Angewandte Chemie (International ed. in English) · 2023Article
- The use of RNA-based treatments in the field of cancer immunotherapy.Molecular cancer · 2023Review
- Neural modulation with photothermally active nanomaterials.Nature reviews bioengineering · 2023Article
- Gold and Carbon-Based Nano-theranostics: An Overview on the Developments and Applications for Cancer Phototherapy.Advanced pharmaceutical bulletin · 2022Review
- Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene.International journal of molecular sciences · 2021Article
- Design and Encapsulation of Immunomodulators onto Gold Nanoparticles in Cancer Immunotherapy.International journal of molecular sciences · 2021Review
- Skeletal Stem Cells as the Developmental Origin of Cellular Niches for Hematopoietic Stem and Progenitor Cells.Current topics in microbiology and immunology · 2021Article
- Heat-Triggered Remote Control of CRISPR-dCas9 for Tunable Transcriptional Modulation.ACS chemical biology · 2020Article
- Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Synthetic immunity by remote control.Theranostics · 2020Review
- Gold nanoparticles in chemo-, immuno-, and combined therapy: review [Invited].Biomedical optics express · 2019Article
- Gold nanorods together with HSP inhibitor-VER-155008 micelles for colon cancer mild-temperature photothermal therapy.Acta pharmaceutica Sinica. B · 2018Article
- Remote Control of Mammalian Cells with Heat-Triggered Gene Switches and Photothermal Pulse Trains.ACS synthetic biology · 2018Article
- Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods.Scientific reports · 2017Article
- Gold nanoparticle-mediated photothermal therapy: applications and opportunities for multimodal cancer treatment.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2017Review
- Photoactivatable RNAi for cancer gene therapy triggered by near-infrared-irradiated single-walled carbon nanotubes.International journal of nanomedicine · 2017Article
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- Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Modulation of the cytokine milieu is one approach for vaccine development. However, therapy with pro-inflammatory cytokines, such as IL-12, is limited in practice due to adverse systemic effects. Spatially-restricted gene expression circumvents this problem by enabling localized amplification. Intracellular co-delivery of gold nanorods (AuNR) and a heat shock protein 70 (HSP70) promoter-driven expression vector enables gene expression in response to near infrared (NIR) light. AuNRs absorb the light, convert it into heat and thereby stimulate photothermal expression of the cytokine. As proof-of-concept, human HeLa and murine B16 cancer cells were transfected with a HSP70-Enhanced Green Fluorescent Protein (EGFP) plasmid and polyethylenimine (PEI)-conjugated AuNRs. Exposure to either 42 °C heat-shock or NIR light induced significant expression of the reporter gene. In vivo NIR driven expression of the reporter gene was confirmed at 6 and 24 h in mice bearing B16 melanoma tumors using in vivo imaging and flow-cytometric analysis. Overall, we demonstrate a novel opportunity for site-directed, heat-inducible expression of a gene based upon the NIR-absorbing properties of AuNRs and a HSP70 promoter-driven expression vector.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.