Evidence map›Paper›PMID 25327529›Full record

ArticleJournal of lipid research2015

Regulation of NF-κB signaling by oxidized glycerophospholipid and IL-1β induced miRs-21-3p and -27a-5p in human aortic endothelial cells.

Milagros C Romay, Nam Che, Scott N Becker, Delila Pouldar, Raffi Hagopian, Xinshu Xiao, Aldons J Lusis, Judith A Berliner, Mete Civelek

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of lipid research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 42 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. The role of microRNAs in acrylamide toxicity.Frontiers in nutrition · 2024
    Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Increased miR-21-3p and miR-487b-3p serum levels during anaphylactic reaction in food allergic children.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Milagros C RomayDepartments of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095.
Nam CheMedicine, University of California, Los Angeles, Los Angeles, CA 90095.
Scott N BeckerDepartments of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095.
Delila PouldarMedicine, University of California, Los Angeles, Los Angeles, CA 90095.
Raffi HagopianHuman Genetics, University of California, Los Angeles, Los Angeles, CA 90095.
Xinshu XiaoIntegrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA 90095.
Aldons J LusisDepartments of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 Medicine, University of California, Los Angeles, Los Angeles, CA 90095 Human Genetics, University of California, Los Angeles, Los Angeles, CA 90095.
Judith A BerlinerPathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA 90095.
Mete CivelekMedicine, University of California, Los Angeles, Los Angeles, CA 90095.
University of California, Los Angeles · US

Funding

ULTRASTRUCTURE OF THE INTIMA IN EARLY LESION FORMATIONP01HL030568 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FOGELMAN, ALAN M · 1985 to 2019
$55.2M
Systems genomics of metabolic syndrome traitsP01HL028481 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PAJUKANTA, PAIVI · 1985 to 2019
$50.2M
UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Vascular Biology Training GrantT32HL069766 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LUSIS, ALDONS JAKE · 2002 to 2022
$7.4M
Systems genetics analysis of cardiometabolic trait loci in humans R00HL121172 · NHLBI · UNIVERSITY OF VIRGINIA · PI CIVELEK, METE · 2016 to 2018
$741k
Systems genetics analysis of cardiometabolic trait loci in humans K99HL121172 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CIVELEK, METE · 2014 to 2015
$247k
NHLBI NIH HHS HL-28481NHLBI NIH HHS HL-30568NHLBI NIH HHS K99 HL121172NHLBI NIH HHS K99HL121172NHLBI NIH HHS P01 HL028481NHLBI NIH HHS P01 HL030568NHLBI NIH HHS R00 HL121172NHLBI NIH HHS T32 HL069766NHLBI NIH HHS T32HL69766NIGMS NIH HHS T32 GM007185NIGMS NIH HHS T32GM718539
6 · The paper itself

Abstract

Exposure of endothelial cells (ECs) to agents such as oxidized glycerophospholipids (oxGPs) and cytokines, known to accumulate in atherosclerotic lesions, perturbs the expression of hundreds of genes in ECs involved in inflammatory and other biological processes. We hypothesized that microRNAs (miRNAs) are involved in regulating the inflammatory response in human aortic endothelial cells (HAECs) in response to oxGPs and interleukin 1β (IL-1β). Using next-generation sequencing and RT-quantitative PCR, we characterized the profile of expressed miRNAs in HAECs pre- and postexposure to oxGPs. Using this data, we identified miR-21-3p and miR-27a-5p to be induced 3- to 4-fold in response to oxGP and IL-1β treatment compared with control treatment. Transient overexpression of miR-21-3p and miR-27a-5p resulted in the downregulation of 1,253 genes with 922 genes overlapping between the two miRNAs. Gene Ontology functional enrichment analysis predicted that the two miRNAs were involved in the regulation of nuclear factor κB (NF-κB) signaling. Overexpression of these two miRNAs leads to changes in p65 nuclear translocation. Using 3' untranslated region luciferase assay, we identified 20 genes within the NF-κB signaling cascade as putative targets of miRs-21-3p and -27a-5p, implicating these two miRNAs as modulators of NF-κB signaling in ECs.

Indexed as

3' Untranslated RegionsActive Transport, Cell NucleusCell NucleusEndothelial CellsGene Expression RegulationHumansInterleukin-1betaMicroRNAsOxidation-ReductionPhosphatidylcholinesSequence Analysis, RNASignal TransductionTranscription Factor RelATumor Necrosis Factor-alpha1-palmitoyl-2-arachidonyl-3-phosphorylcholine3' Untranslated RegionsInterleukin-1betaMicroRNAsMIRN21 microRNA, humanMIRN27 microRNA, humanPhosphatidylcholinesTranscription Factor RelATumor Necrosis Factor-alphacell signalingcytokinesgene expressioninflammation oxidized lipidsinterleukin 1βmicroRNAsnuclear factor κB

Identifiers

PMID25327529
PMCPMC4274069
OpenAlexW2090537785

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.